EVALUATION OF HOUSEKEEPING GENE EXPRESSION OF WHEAT INTERACTION AGAINST MYCOSPHAERELLA GRAMINICOLAWITH REVERSE NORTHERN DOT BLOT METHOD

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GHOLAMNEZHAD JALAL; SANJARIAN FOROUGH; MOHAMMADI GOLTAPEH EBRAHIM; SAFAEI NASER; RAZAVI KHADIJEH

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Journal: Crop Biotechnology Year:2016 | Volume:4 | Issue:12 Page(s): 1-10

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Title

EVALUATION OF HOUSEKEEPING GENE EXPRESSION OF WHEAT INTERACTION AGAINST MYCOSPHAERELLA GRAMINICOLAWITH REVERSE NORTHERN DOT BLOT METHOD

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Keywords

GENE EXPRESSION ANALYSISQ2

REFERENCE GENESQ2

WHEAT-M. GRAMINICOLAQ2

REVERSE NORTHERN DOT BLOTQ2

Abstract

As a rule the REFERENCE GENES used in GENE EXPRESSION ANALYSIS have been selected for their housekeeping roles, but the variation observed in most of them is a major obstacle to their effective use. It is widely supported to identify and validate stable REFERENCE GENES, since no single biological gene is stably expressed between cell types or within cells under different conditions. In this study, suitability of seven wheat housekeeping genes for normalization of mRNA expression in wheat leaves infected by Mycosphaerella graminicolawas investigated. Expression level of Actin, Rubisco, Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Translation Elongation Factor 1α (TEF- 1a), a-Tubollin, eukaryotic release factors 1 and 3 (ERF 1 and ERF 3) genes were examined by REVERSE NORTHERN DOT BLOT method. Expression stabilities of the REFERENCE GENES were statistically analyzed by Ecxel and SAS softwares. a-Tubolin, TEF- 1a and Actin were the three most stable genes whereas the expression of Rubisco and GAPDH had the least stability. The presented comprehensive data on changes in expression of various wheat housekeeping genes in WHEAT-M. GRAMINICOLA phatosystem facilitate selection of REFERENCE GENES for REVERSE NORTHERN DOT BLOT method.

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APA: Copy

GHOLAMNEZHAD, JALAL, SANJARIAN, FOROUGH, MOHAMMADI GOLTAPEH, EBRAHIM, SAFAEI, NASER, & RAZAVI, KHADIJEH. (2016). EVALUATION OF HOUSEKEEPING GENE EXPRESSION OF WHEAT INTERACTION AGAINST MYCOSPHAERELLA GRAMINICOLAWITH REVERSE NORTHERN DOT BLOT METHOD. JOURNAL OF CROP BIOTECHNOLOGY, 4(12), 1-10. SID. https://sid.ir/paper/226776/en

Vancouver: Copy

GHOLAMNEZHAD JALAL, SANJARIAN FOROUGH, MOHAMMADI GOLTAPEH EBRAHIM, SAFAEI NASER, RAZAVI KHADIJEH. EVALUATION OF HOUSEKEEPING GENE EXPRESSION OF WHEAT INTERACTION AGAINST MYCOSPHAERELLA GRAMINICOLAWITH REVERSE NORTHERN DOT BLOT METHOD. JOURNAL OF CROP BIOTECHNOLOGY[Internet]. 2016;4(12):1-10. Available from: https://sid.ir/paper/226776/en

IEEE: Copy

JALAL GHOLAMNEZHAD, FOROUGH SANJARIAN, EBRAHIM MOHAMMADI GOLTAPEH, NASER SAFAEI, and KHADIJEH RAZAVI, “EVALUATION OF HOUSEKEEPING GENE EXPRESSION OF WHEAT INTERACTION AGAINST MYCOSPHAERELLA GRAMINICOLAWITH REVERSE NORTHERN DOT BLOT METHOD,” JOURNAL OF CROP BIOTECHNOLOGY, vol. 4, no. 12, pp. 1–10, 2016, [Online]. Available: https://sid.ir/paper/226776/en

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