INDUCTION OF GYNOGENESIS IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) BY DV-IRRADIATION

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AZARI TAKAMI GHOBAD; FARAHMAND H.; BAHRAMI KAMANGAR B.

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Journal: IRANIAN JOURNAL OF NATURAL RESOURCES Year:2002 | Volume:54 | Issue:4 Page(s): 369-382

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Title

INDUCTION OF GYNOGENESIS IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) BY DV-IRRADIATION

Author(s)

AZARI TAKAMI GHOBAD | FARAHMAND H. | BAHRAMI KAMANGAR B. | Issue Writer Certificate

Keywords

GYNOGENESISQ4

SPERMATOZOAQ4

RAINBOW TROUTQ4

DV-IRRADIATIONQ4

GENOMEQ4

Abstract

In order to induce meiosis GYNOGENESIS in RAINBOW TROUT (Oncorhynchus mykiss), SPERMATOZOA GENOME was destroyed by Uv irradiation (2887 µw/cm2) and after fertilization with intact ovum, diploidy was returned by applying a thermal shock (27±0.5°c) for 10 minutes. Thermal shock was applied at 30 and 40 minutes after fertilization. Uv-irradiation was supplied from a germicidal lamp (30 w) with maximum intensity at 245 nm.Before irradiation; sperm was diluted with two kinds of solutions at a ratio of 1:4 and then subjected to 1,3,5,8 and 15 minutes of irradiation. Statistical design for comprising effects on 3 different treatment groups were observed as split split plot on GYNOGENESIS and split plot on haploid and randomized complete block on control. Golden color in RAINBOW TROUT was used as a marker for distinguishing gynogenetic offspring. Furthemore, for confirmed diploidy chromosome slides were prepared. The best performance was achieved in treatment of 8 minute irradiation and applied thermal shock of 30 minutes after fertilization, but there was no significant difference between the two solutions (P>0.05). The mean percentage GYNOGENESIS was 60.61% in the treatments of three replications. Nevertheless, 1000/0 GYNOGENESIS was achieved from 8 minute irradiation in the second replication.

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بررسی ایجاد تغییر جنسیت و عقیمی در ماهی کپور معمولی (Cyprinus carpio Linne) به وسیله هورمون 17 آلفا - متیل تستوسترون

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APA: Copy

AZARI TAKAMI, GHOBAD, FARAHMAND, H., & BAHRAMI KAMANGAR, B.. (2002). INDUCTION OF GYNOGENESIS IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) BY DV-IRRADIATION. IRANIAN JOURNAL OF NATURAL RESOURCES, 54(4), 369-382. SID. https://sid.ir/paper/23119/en

Vancouver: Copy

AZARI TAKAMI GHOBAD, FARAHMAND H., BAHRAMI KAMANGAR B.. INDUCTION OF GYNOGENESIS IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) BY DV-IRRADIATION. IRANIAN JOURNAL OF NATURAL RESOURCES[Internet]. 2002;54(4):369-382. Available from: https://sid.ir/paper/23119/en

IEEE: Copy

GHOBAD AZARI TAKAMI, H. FARAHMAND, and B. BAHRAMI KAMANGAR, “INDUCTION OF GYNOGENESIS IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) BY DV-IRRADIATION,” IRANIAN JOURNAL OF NATURAL RESOURCES, vol. 54, no. 4, pp. 369–382, 2002, [Online]. Available: https://sid.ir/paper/23119/en

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