Investigation of Immunization of DNA-Based Polyepitop HIV Vaccine Candidate in Mouse Model and the Impact of Alum Adjuvant and Subcutaneous Infusion on its Efficiency

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Jazaeri E.O.; MAHDAVI A.; ABDOLI A.

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Journal: JOURNAL OF BIOTECHNOLOGY Year:2019 | Volume:10 | Issue:1 Page(s): 133-141

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Title

Investigation of Immunization of DNA-Based Polyepitop HIV Vaccine Candidate in Mouse Model and the Impact of Alum Adjuvant and Subcutaneous Infusion on its Efficiency

Author(s)

Jazaeri E.O. | MAHDAVI A. | ABDOLI A. | Issue Writer Certificate

Keywords

AIDSQ2

DNA VaccineQ1

AdjuvantQ2

Cellular ImmunityQ2

Humoral ImmunityQ1

Abstract

Aims One of the challenges of today’ s world and also global health priorities is pandemicity of AIDS. Studies have shown that the scope and breadth of the immune responses induction are very effective to protect against HIV. Moreover, simultaneous induction of humoral and Cellular Immunity responses increases the effectiveness of candidate HIV vaccines. To attain these goals, new approaches such as polyepitopic vaccine strategy and addition of different Adjuvants in HIV vaccines’ formulations have been recently considered. Materials & Methods In the present study, eukaryotic expression vector (pcDNA3. 1-tat/pol/ gag/env) was transformed and amplified in the prokaryotic host cells E. coli (DH5α ). After vector extraction, it was concentrated and formulated alone and in combination with Alum Adjuvant and used as DNA candidate vaccines. DNA candidate vaccines were, then, subcutaneously injected to the BALB/c mice on 0, 14, and 28 days and elicited humoral and Cellular Immunity responses were finally evaluated. Findings The results showed that the candidate DNA Vaccine could not efficiently induce immunity responses (both humoral and cellular responses) by subcutaneous route injection. Conclusion Maybe, in one hand, pcDNA3. 1-tat/pol/gag/env vector could not effectively be taken by APC and subsequently HIV1-top4 antigens could not be expressed, processed, and presented. On the other hand, it is likely that inefficiency subcutaneous injection leads to these results. Therefore, other vaccines’ injection and deliveries routes along with addition of other Adjuvants in vaccine’ s formulations could induce immunity responses efficiently and increase vaccine efficacy.

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APA: Copy

Jazaeri, E.O., MAHDAVI, A., & ABDOLI, A.. (2019). Investigation of Immunization of DNA-Based Polyepitop HIV Vaccine Candidate in Mouse Model and the Impact of Alum Adjuvant and Subcutaneous Infusion on its Efficiency. JOURNAL OF BIOTECHNOLOGY, 10(1 ), 133-141. SID. https://sid.ir/paper/231239/en

Vancouver: Copy

Jazaeri E.O., MAHDAVI A., ABDOLI A.. Investigation of Immunization of DNA-Based Polyepitop HIV Vaccine Candidate in Mouse Model and the Impact of Alum Adjuvant and Subcutaneous Infusion on its Efficiency. JOURNAL OF BIOTECHNOLOGY[Internet]. 2019;10(1 ):133-141. Available from: https://sid.ir/paper/231239/en

IEEE: Copy

E.O. Jazaeri, A. MAHDAVI, and A. ABDOLI, “Investigation of Immunization of DNA-Based Polyepitop HIV Vaccine Candidate in Mouse Model and the Impact of Alum Adjuvant and Subcutaneous Infusion on its Efficiency,” JOURNAL OF BIOTECHNOLOGY, vol. 10, no. 1 , pp. 133–141, 2019, [Online]. Available: https://sid.ir/paper/231239/en

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