Optimization of Direct Regeneration Method in Ziziphus jujuba Mill

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Hashemzadeh Z.; JAFARKHANI KERMANI M.; HAMIDOGHLI Y.

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Journal: JOURNAL OF BIOTECHNOLOGY Year:2018 | Volume:9 | Issue:4 Page(s): 495-500

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Title

Optimization of Direct Regeneration Method in Ziziphus jujuba Mill

Author(s)

Hashemzadeh Z. | JAFARKHANI KERMANI M. | HAMIDOGHLI Y. | Issue Writer Certificate

Keywords

RegenerationQ2

LeafQ2

Growth RegulatorQ2

Ziziphus jujubeQ1

Tissue CultureQ2

Abstract

Aims The mass propagation and breeding new varieties of Ziziphus jujuba Mill as a valuable fruit tree and herb, which is well adapted to dry and semi-arid climate, is very important. The aim of the present research was to optimize direct Regeneration method in Ziziphus jujuba Mill. Materials & Methods In this experimental study, the explants consisted of Leaf cut into 3 parts, Leaf cut from 4 sides, and full Leaf of in vitro and compared in Murashige and Skoog and woody plant media with different concentrations of Thidiazuron (TDZ; 0, 5, 10, 15, and 20μ M) and Naphthalene acetic acid (NAA, 0 and 1μ M). The effect of 2 and 4 weeks of darkness on Regeneration rate was investigated. The experiments were conducted in factorial based on a completely randomized design. The mean of statistical data was compared using Duncan’ s multiple range test. SAS 9. 3. 1 software was used and the difference was considered significant at 1% probability level. Findings The 2 weeks of darkness treatment with the mean of 1. 38 was better than the 4 weeks of darkness treatment. The Maximum number of shoots (2. 27) was obtained in Leaf cut into 3 parts. The maximum percent of Regeneration (75. 0%) and highest number of regenerated shoots (4. 83) were obtained in the MS medium containing 10μ Μ TDZ and 1μ Μ NAA. Conclusion Regeneration rates in Ziziphus jujuba Mill is affected by the type of explant, culture media and plant Growth Regulators. Maximum rate of Regeneration is observed in Leaf cut into 3 parts and cultured on MS medium containing 1μ M NAA and 10μ Μ TDZ. Plantlets are rooted and successfully acclimatized at “ in vivo” conditions.

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APA: Copy

Hashemzadeh, Z., JAFARKHANI KERMANI, M., & HAMIDOGHLI, Y.. (2018). Optimization of Direct Regeneration Method in Ziziphus jujuba Mill. JOURNAL OF BIOTECHNOLOGY, 9(4 ), 495-500. SID. https://sid.ir/paper/231306/en

Vancouver: Copy

Hashemzadeh Z., JAFARKHANI KERMANI M., HAMIDOGHLI Y.. Optimization of Direct Regeneration Method in Ziziphus jujuba Mill. JOURNAL OF BIOTECHNOLOGY[Internet]. 2018;9(4 ):495-500. Available from: https://sid.ir/paper/231306/en

IEEE: Copy

Z. Hashemzadeh, M. JAFARKHANI KERMANI, and Y. HAMIDOGHLI, “Optimization of Direct Regeneration Method in Ziziphus jujuba Mill,” JOURNAL OF BIOTECHNOLOGY, vol. 9, no. 4 , pp. 495–500, 2018, [Online]. Available: https://sid.ir/paper/231306/en

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