PREVALENCE OF LEPTOSPIRA SPP. IN BOVINE ABORTED FETUSES OF DAIRY CATTLE HERDS BY PCR IN TEHRAN PROVINCE

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BADIEE A.; MOUSAKHANI F.; MALEKAN M.; ZAFARI M.

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Journal: JOURNAL OF VETERINARY CLINICAL RESEARCH Year:2010 | Volume:1 | Issue:3 (3) Page(s): 153-160

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Title

PREVALENCE OF LEPTOSPIRA SPP. IN BOVINE ABORTED FETUSES OF DAIRY CATTLE HERDS BY PCR IN TEHRAN PROVINCE

Author(s)

BADIEE A. | MOUSAKHANI F. | MALEKAN M. | ZAFARI M. | Issue Writer Certificate

Keywords

LEPTOSPIROSISQ3

ABORTIONQ3

PCRQ2

INFERTILITYQ2

REDUCED MILK PRODUCTIONQ3

Abstract

LEPTOSPIROSIS is a zoonotic disease that can cause heavy economic losses through complications including ABORTION, stillbirth, INFERTILITY, REDUCED MILK PRODUCTION, etc. In this study, 251 referred samples of aborted fetuses in industrial dairy herds of Tehran province evaluated in MABNA laboratory during one year. Tested samples included a homogenous tissue mixture of fatal heart, kidney, liver and spleen. For DNA extraction, the Proteinase K Method was used. Then polymerase chain reaction (PCR) was performed to recognize Leptospira Spp. by using specific 16s rRNA gene primers. Samples with 331bp band in electrophoresis gel were considered positive. Prevalence of leptospiral ABORTION in average was 12.8% in Tehran province during a year and based on season, respectively, the amount in spring was 15%, summer 9.08%, autumn 10% and winter 19.2%. Based on these results, it was most prevalent in spring and winter that is probably due to higher environmental humidity, hydrophilic nature of leptospira and more bacterial activities. Generally according to the prevalence of 12.8%, the major cause of ABORTION in Tehran province in industrial dairy herds is leptospiral infection so that any activities in order to eradicate and control the disease is essential.

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APA: Copy

BADIEE, A., MOUSAKHANI, F., MALEKAN, M., & ZAFARI, M.. (2010). PREVALENCE OF LEPTOSPIRA SPP. IN BOVINE ABORTED FETUSES OF DAIRY CATTLE HERDS BY PCR IN TEHRAN PROVINCE. JOURNAL OF VETERINARY CLINICAL RESEARCH, 1(3 (3)), 153-160. SID. https://sid.ir/paper/233007/en

Vancouver: Copy

BADIEE A., MOUSAKHANI F., MALEKAN M., ZAFARI M.. PREVALENCE OF LEPTOSPIRA SPP. IN BOVINE ABORTED FETUSES OF DAIRY CATTLE HERDS BY PCR IN TEHRAN PROVINCE. JOURNAL OF VETERINARY CLINICAL RESEARCH[Internet]. 2010;1(3 (3)):153-160. Available from: https://sid.ir/paper/233007/en

IEEE: Copy

A. BADIEE, F. MOUSAKHANI, M. MALEKAN, and M. ZAFARI, “PREVALENCE OF LEPTOSPIRA SPP. IN BOVINE ABORTED FETUSES OF DAIRY CATTLE HERDS BY PCR IN TEHRAN PROVINCE,” JOURNAL OF VETERINARY CLINICAL RESEARCH, vol. 1, no. 3 (3), pp. 153–160, 2010, [Online]. Available: https://sid.ir/paper/233007/en

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