DETERMINATION OF DIAGNOSTIC VALUE OF MILK AMYLOID A AS A NEW BIOMARKER OF BULK MILK QUALITY

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TAGHDIRI M.R.; KARIM G.; SAFI S.; RAHIMI FROUSHANI A.; MOTALEBI A.A.

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Journal: JOURNAL OF VETERINARY CLINICAL RESEARCH Year:2013 | Volume:4 | Issue:2 (14) Page(s): 71-90

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Title

DETERMINATION OF DIAGNOSTIC VALUE OF MILK AMYLOID A AS A NEW BIOMARKER OF BULK MILK QUALITY

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Keywords

MILK AMYLOID AQ2

BIOMARKERQ2

MILK QUALITYQ2

Abstract

Somatic cell count (SCC) has been considered as the golden standard method to evaluate raw bulk MILK QUALITY for several decades. Nevertheless, SCC is neither sensitive nor specific enough to evaluate raw bulk milk. Much effort has been performed to substitute new markers in this regard. Acute phase proteins (APPs) as part of inflammatory response received too much attention as new diagnostic markers in veterinary medicine as well as subclinical mastitis in dairy cattle. Since subclinical mastitis cause unfavorable changes in milk composition, APPs would be a potential BIOMARKERs to predict MILK QUALITY. The objectives of this study were to determine the diagnostic value of MILK AMYLOID A (MAA) for evaluating raw bulk MILK QUALITY delivered to dairy industry and to establish cut off points to detect early changes in bulk tank MILK QUALITY. The study performed on 30 random selected dairy farms delivering their bulk milk to Pegah dairy industries at Tehran province. Three milk samples collected at 3 different times to measure MAA, SCC, total protein, casein, total fat and lactose level using commercially available methods. Diagnostic sensitivity and specificity and cutoff points for each test were determined using receiver-operating characteristics (ROC) analysis. MAA was the most accurate test with a diagnostic sensitivity of 96% and specificity of 60% at cutoff point of 55.64ng/mL based on a SCC of 200×1000 cells/mL of bulk milk. The study showed that determination of MAA can be used as a potential BIOMARKER to discriminate early unfavorable compositional change in bulk MILK QUALITY based on SCC higher than 200×1000 cells/mL of bulk milk. A significant relationship found between MAA and bulk milk protein quality traits (P<0.05).

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APA: Copy

TAGHDIRI, M.R., KARIM, G., SAFI, S., RAHIMI FROUSHANI, A., & MOTALEBI, A.A.. (2013). DETERMINATION OF DIAGNOSTIC VALUE OF MILK AMYLOID A AS A NEW BIOMARKER OF BULK MILK QUALITY. JOURNAL OF VETERINARY CLINICAL RESEARCH, 4(2 (14)), 71-90. SID. https://sid.ir/paper/233014/en

Vancouver: Copy

TAGHDIRI M.R., KARIM G., SAFI S., RAHIMI FROUSHANI A., MOTALEBI A.A.. DETERMINATION OF DIAGNOSTIC VALUE OF MILK AMYLOID A AS A NEW BIOMARKER OF BULK MILK QUALITY. JOURNAL OF VETERINARY CLINICAL RESEARCH[Internet]. 2013;4(2 (14)):71-90. Available from: https://sid.ir/paper/233014/en

IEEE: Copy

M.R. TAGHDIRI, G. KARIM, S. SAFI, A. RAHIMI FROUSHANI, and A.A. MOTALEBI, “DETERMINATION OF DIAGNOSTIC VALUE OF MILK AMYLOID A AS A NEW BIOMARKER OF BULK MILK QUALITY,” JOURNAL OF VETERINARY CLINICAL RESEARCH, vol. 4, no. 2 (14), pp. 71–90, 2013, [Online]. Available: https://sid.ir/paper/233014/en

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