A STUDY ON EFFECT OF DIFFERENT CULTURE MEDIA ON AMYLASE ENZYME PRODUCTION BY A NATIVE STRAIN OF BACILLUS SUBTILIS

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AKBARI ZIBA; NAYERI HASHEM; BEHESHTIMAAL KEYVAN

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Journal: Journal of Microbial Biology Year:2015 | Volume:4 | Issue:15 Page(s): 99-108

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Title

A STUDY ON EFFECT OF DIFFERENT CULTURE MEDIA ON AMYLASE ENZYME PRODUCTION BY A NATIVE STRAIN OF BACILLUS SUBTILIS

Author(s)

AKBARI ZIBA | NAYERI HASHEM | BEHESHTIMAAL KEYVAN | Issue Writer Certificate

Keywords

AMYLASEQ2

BACILLUS SUBTILISQ2

CULTURE MEDIUMQ2

WASTEWATERQ2

Abstract

Introduction: AMYLASEs are among the most important enzymes and have great significance in present-day biotechnology. AMYLASE with commercial applications is mainly derived from the genus Bacillus. The main purpose of this study is identification and isolatation AMYLASE enzyme producer Bacillus, determining the AMYLASE enzyme activity and affecting a number of CULTURE MEDIUM on AMYLASE enzyme production.Materials and methods: Soil, water and WASTEWATER samples were collected from agricultural area, choghakhor lake in chahar mahal e bakhtiari province and from food factory in Esfahan. Bacillus isolates were screened for amylolytic properties by starch hydrolysis test on starch agar plate. AMYLASE producing Bacillus were identified biochemical tests and molecular experiments. AMYLASE enzyme activity of isolates was measured using di-nitro salicylic acid (DNS) method. Enzyme production was studied in variose medium culture TSB, NB, Yeast extract, molases and milk medium.Results: The enzyme AMYLASE-producing strains, one sample showed was the highest AMYLASE activity. The Bacillus has been detected as a member of BACILLUS SUBTILIS according to Bergey's Manual of Systematic Bacteriology and molecular recognition. The enzyme activity of BACILLUS SUBTILIS was measured 7.21 (U/ml) in production media. Trough medium culture maximum AMYLASE production for BACILLUS SUBTILIS was achieved in molases medium.Discussion and conclusion: In this study, BACILLUS SUBTILIS strains isolated from WASTEWATER of a significant amount of enzyme producing 7.21 (U/ml) as indicated. Among the medium-AMYLASE from BACILLUS SUBTILIS highest enzyme activity was observed in beet molasses. According to this study, the use of Bacillus strains is an efficient way to achieve the AMYLASE enzyme.

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APA: Copy

AKBARI, ZIBA, NAYERI, HASHEM, & BEHESHTIMAAL, KEYVAN. (2015). A STUDY ON EFFECT OF DIFFERENT CULTURE MEDIA ON AMYLASE ENZYME PRODUCTION BY A NATIVE STRAIN OF BACILLUS SUBTILIS. BIOLOGICAL JOURNAL OF MICROORGANISM, 4(15), 99-108. SID. https://sid.ir/paper/237385/en

Vancouver: Copy

AKBARI ZIBA, NAYERI HASHEM, BEHESHTIMAAL KEYVAN. A STUDY ON EFFECT OF DIFFERENT CULTURE MEDIA ON AMYLASE ENZYME PRODUCTION BY A NATIVE STRAIN OF BACILLUS SUBTILIS. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2015;4(15):99-108. Available from: https://sid.ir/paper/237385/en

IEEE: Copy

ZIBA AKBARI, HASHEM NAYERI, and KEYVAN BEHESHTIMAAL, “A STUDY ON EFFECT OF DIFFERENT CULTURE MEDIA ON AMYLASE ENZYME PRODUCTION BY A NATIVE STRAIN OF BACILLUS SUBTILIS,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 4, no. 15, pp. 99–108, 2015, [Online]. Available: https://sid.ir/paper/237385/en

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