SYNTHESIS OF POLY (3-HYDROXYBUTYRATE) USING ENZYMES AZOSPIRILLUM BRASILENSE IN VITRO

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ABBASI SOHEILA; EMTIAZI GITI; ROGHANIAN RASOUL

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Microbial Biology Year:2017 | Volume:6 | Issue:22 Page(s): 127-142

Download Full-Text

Persian Verion

View:

1,716

Download:

Cites:

Information Journal Paper

Title

SYNTHESIS OF POLY (3-HYDROXYBUTYRATE) USING ENZYMES AZOSPIRILLUM BRASILENSE IN VITRO

Author(s)

ABBASI SOHEILA | EMTIAZI GITI | ROGHANIAN RASOUL | Issue Writer Certificate

Keywords

PHBQ1

POLYESTERQ1

AZOSPIRILCOMPARATIVE PRODUCTION OF CELLULASES BY MUTANTS OF TRICHODERMA PARCERAMOSUME PTCC5140Q1

Abstract

Introduction: Polyhydroxyalkaniates (PHAS) are bacterial polymers that are formed as naturally occurring storage POLYESTERs by a wide range of microorganisms usually under unbalanced growth conditions. Mechanical properties of PHAS make them suitable replacements for petrochemically produced bulk plastics (polyethylene, polypropylene etc.), but in contrast to these commodity plastics PHA are completely degradable to carbon dioxide and water through natural microbiological mineralization. PHAS can be produced by biotechnological processes under controlled conditions. Polyhydroxybotyric acid (PHB) was the first of the PHAS discovered and is the most abundant POLYESTER found in bacteria.Materials and methods: First, a rich bacterial suspension prepared from poly(3-hydroxybutyrate), then the cells were broken down and ensure the absence of live cells. The cell free enzymes were added to M9 medium. Then the sample turbidity was measured at a wavelength of 600 nm and incubated at 30°C and centrifiuged at 120rpm. Next, the amount of PHB as well as solution turbidity were measured and compared with control. The physical and chemical analyzes of extracted PHB samples such as UV absorbtion, FTIR, HPLC and HNMR assesed and compared with standard sample.Results: In twelve days this experiment was conducted, from the third day, the amount of polyhydroxybutyrate was measurable and the highest polyhydroxybutyrate was gained on the tenth day. The level of PHB was constant. Moreover, the level of glucose was decreased gradually till the tenth day. By Physico-chemical analysis, production of polyhydroxybutyrate was approved.Discussion and conclusion: In this experiment, the reaction efficiency was 37.5 percent. Probably the rest of glucose could be changed to other intermediate compounds by other enzymes in the sample. Therefore, by purification of the enzyme, usage of specific substrate and optimization of conditions outside the cell could help the production of polyhydroxybutyrate continually. Production is very cost-effective, by this method.

Cites

No record.

References

No record.

Cite

APA: Copy

ABBASI, SOHEILA, EMTIAZI, GITI, & ROGHANIAN, RASOUL. (2017). SYNTHESIS OF POLY (3-HYDROXYBUTYRATE) USING ENZYMES AZOSPIRILLUM BRASILENSE IN VITRO. BIOLOGICAL JOURNAL OF MICROORGANISM, 6(22 ), 127-142. SID. https://sid.ir/paper/237412/en

Vancouver: Copy

ABBASI SOHEILA, EMTIAZI GITI, ROGHANIAN RASOUL. SYNTHESIS OF POLY (3-HYDROXYBUTYRATE) USING ENZYMES AZOSPIRILLUM BRASILENSE IN VITRO. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2017;6(22 ):127-142. Available from: https://sid.ir/paper/237412/en

IEEE: Copy

SOHEILA ABBASI, GITI EMTIAZI, and RASOUL ROGHANIAN, “SYNTHESIS OF POLY (3-HYDROXYBUTYRATE) USING ENZYMES AZOSPIRILLUM BRASILENSE IN VITRO,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 6, no. 22 , pp. 127–142, 2017, [Online]. Available: https://sid.ir/paper/237412/en

Related Journal Papers