OPTIMIZATIONS OF α-AMYLASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY IN IMMOBILIZATION BACILLUS AMYLOLIQUEFACIENS ATCC 23350

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SAMADLOUIE HAMID REZA; KARIMIROOZBAHANI MARYAM; KABOOSI HAMI; FARROKHI NASER

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Journal: Journal of Microbial Biology Year:2016 | Volume:4 | Issue:16 Page(s): 77-86

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Title

OPTIMIZATIONS OF α-AMYLASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY IN IMMOBILIZATION BACILLUS AMYLOLIQUEFACIENS ATCC 23350

Author(s)

SAMADLOUIE HAMID REZA | KARIMIROOZBAHANI MARYAM | KABOOSI HAMI | FARROKHI NASER | Issue Writer Certificate

Keywords

BACILLUS AMYLOLIQUEFACIENSQ2

ENZYME PRODUCTIONQ2

FERMENTATIONQ2

IMMOBILIZATIONQ2

RESPONSE SURFACE METHODOLOGYQ1

Abstract

Introduction: Production of an endogenous α-amylase from BACILLUS AMYLOLIQUEFACIENS ATCC 23350 was studied and enhanced.Materials and methods: Protein and carbon sources were analyzed for free and immobilized bacterial cells and number of beads was considered for immobilized cells via one factor at a time methodforα-amylase production by BACILLUS AMYLOLIQUEFACIENS. Subsequently, optimization condition was employed solely for immobilized bacterial cells by RESPONSE SURFACE METHODOLOGY (RSM).Results: Peptone and rice starch showed to improve the α-amylase production in immobilized Bacillus cells. RSM generated a mathematical model explaining the optimum concentration of the efficient nutrients (139.35 g/l of rice starch and 80.00 g/l of peptone) leading to an optimum amylase production (205 U/ml).Discussion and conclusion: The statistical advance displayed significant outcomes to optimize the process parameters for maximal α-amylase production using BACILLUS AMYLOLIQUEFACIENS and gave permission to rapid screening of variables. RSM led to find out an immense improvement in enzyme activity (more than 90%: from 25 to 225 U/ml) for the first time.

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APA: Copy

SAMADLOUIE, HAMID REZA, KARIMIROOZBAHANI, MARYAM, KABOOSI, HAMI, & FARROKHI, NASER. (2016). OPTIMIZATIONS OF α-AMYLASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY IN IMMOBILIZATION BACILLUS AMYLOLIQUEFACIENS ATCC 23350. BIOLOGICAL JOURNAL OF MICROORGANISM, 4(16), 77-86. SID. https://sid.ir/paper/237463/en

Vancouver: Copy

SAMADLOUIE HAMID REZA, KARIMIROOZBAHANI MARYAM, KABOOSI HAMI, FARROKHI NASER. OPTIMIZATIONS OF α-AMYLASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY IN IMMOBILIZATION BACILLUS AMYLOLIQUEFACIENS ATCC 23350. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2016;4(16):77-86. Available from: https://sid.ir/paper/237463/en

IEEE: Copy

HAMID REZA SAMADLOUIE, MARYAM KARIMIROOZBAHANI, HAMI KABOOSI, and NASER FARROKHI, “OPTIMIZATIONS OF α-AMYLASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY IN IMMOBILIZATION BACILLUS AMYLOLIQUEFACIENS ATCC 23350,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 4, no. 16, pp. 77–86, 2016, [Online]. Available: https://sid.ir/paper/237463/en

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