Effects of Proteinaceous Extract of Rapeseed on the Colorado Potato Beetle (Leptinotarsa decemlineata Say) Digestive Alpha-Amylase Activity and Some Biological Parameters

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ASHOURI SH.; FARSHBAF POURABAD R.

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Journal: Journal of Applied Research in Plant Protection Year:2016 | Volume:5 | Issue:2 Page(s): 199-214

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Title

Effects of Proteinaceous Extract of Rapeseed on the Colorado Potato Beetle (Leptinotarsa decemlineata Say) Digestive Alpha-Amylase Activity and Some Biological Parameters

Author(s)

ASHOURI SH. | FARSHBAF POURABAD R. | Issue Writer Certificate

Keywords

Ammonium sulfate protein precipitationQ1

BioassayQ1

Enzyme inhibition kineticQ2

Leptinotarsa decemlineataQ2

ZymogramQ1

Abstract

Plants have a set of protein molecules that are able to interfere with the digestion of herbivore insects and can be used as an approach to pest control. In this study, digestive alpha-amylase inhibition of Colorado potato beetle, Leptinotarsa decemlineata (Say), by proteinaceous seed extract of rapeseed, Brassica napus L. cv. Karaj3 was investigated. The most inhibitory effect of proteinaceous extracts on the alpha-amylase activity was at pH 5. Four precipitated rapeseed proteinaceous fractions; 0-30, 30-50, 50-70, and 70-100% saturation by ammonium sulfate, caused 0, 52, 12 and 0% inhibition on the gut alpha-amylase activity of the fourth instar larvae, respectively. Fraction 30-50% resulted in 84, 86, 74, 52 and 37% reduction in the L1, L2, L3, L4 and adult’ s gut alpha-amylase activity, respectively. The Enzyme inhibition kinetic test was conducted by the fraction 30-50% on the alpha-amylase of adults and the type of inhibition was determined uncompetitive. In the Zymogram and studying inhibition on the gel, the inhibitory effect was seen as a reduction in intensity of the bands. In Bioassays, different cultivars of potato; Agria, Burren, Picasso and Marx were used. Potato leaves were treated with extract and fed by larvae. No significant difference was observed in the duration of molting from third to fourth-instar larvae on any cultivars. But, weight of fourth-instar larvae was declined on Picasso and Marx and the percentage of fourth instar larval evaluation was decreased on Agria, Picasso and Marx. The alphaamylase activity of fourth instar larvae declined on Agria, significantly compared to the control.

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APA: Copy

ASHOURI, SH., & FARSHBAF POURABAD, R.. (2016). Effects of Proteinaceous Extract of Rapeseed on the Colorado Potato Beetle (Leptinotarsa decemlineata Say) Digestive Alpha-Amylase Activity and Some Biological Parameters. JOURNAL OF APPLIED RESEARCHES IN PLANT PROTECTION, 5(2 ), 199-214. SID. https://sid.ir/paper/237626/en

Vancouver: Copy

ASHOURI SH., FARSHBAF POURABAD R.. Effects of Proteinaceous Extract of Rapeseed on the Colorado Potato Beetle (Leptinotarsa decemlineata Say) Digestive Alpha-Amylase Activity and Some Biological Parameters. JOURNAL OF APPLIED RESEARCHES IN PLANT PROTECTION[Internet]. 2016;5(2 ):199-214. Available from: https://sid.ir/paper/237626/en

IEEE: Copy

SH. ASHOURI, and R. FARSHBAF POURABAD, “Effects of Proteinaceous Extract of Rapeseed on the Colorado Potato Beetle (Leptinotarsa decemlineata Say) Digestive Alpha-Amylase Activity and Some Biological Parameters,” JOURNAL OF APPLIED RESEARCHES IN PLANT PROTECTION, vol. 5, no. 2 , pp. 199–214, 2016, [Online]. Available: https://sid.ir/paper/237626/en

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