Identification of native fungi in contaminated soils of Marun oil field of Ahwaz

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DEHDARI F.; Mehrabi Koushki M.; HAYATI J.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Journal of Soil Biology Year:2019 | Volume:7 | Issue:2 Page(s): 149-165

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Information Journal Paper

Title

Identification of native fungi in contaminated soils of Marun oil field of Ahwaz

Author(s)

DEHDARI F. | Mehrabi Koushki M. | HAYATI J. | Issue Writer Certificate

Keywords

ITS regionQ1

BLASTnQ1

Phylogenetic analysisQ1

Abstract

In this study, 16 contaminated soil samples (with petroleum products) were collected from four different sites at Marun oil field of Ahwaz. Thirty three fungal isolates were isolated and purified using single-spore and hyphal-tip methods. 23 fungal isolates were selected for morphological and molecular identification. The mycelial biomass of the purified fungal isolates was produced into potato-dextrose-broth medium. DNA samples were extracted from freeze-dried mycelia and ITS and Glycerol-3-phosphate dehydrogenase (GPDH, only for Curvularia) regions were amplified using appropriate primers. Amplified products were purified using ethanol precipitation and gel extraction, and sequenced by Macrogen Company. The obtained sequences were edited and assembled and then used in BLASTn search and ML Phylogenetic analysis. Fungal isolates were identified using morphological and molecular studies as follow: Acremonium sclerotigenum, Alternaria destruens, Aspergillus sp., Cladosporium puyae, Curvularia sp., Fusarium sp., Mucor circinelloides, Penicillium oxalicum, P. chrysogenum, Paramyrothecium terrestris, Rhizopus oryzae and the isolates of both Allophoma and Neodidymelliopsis genera from the family Didymellacae. Both the Cladosporium puyae and Paramyrothecium terrestris are new records in IRAN Mycobiota

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APA: Copy

DEHDARI, F., Mehrabi Koushki, M., & HAYATI, J.. (2019). Identification of native fungi in contaminated soils of Marun oil field of Ahwaz. JOURNAL OF SOIL BIOLOGY, 7(2 ), 149-165. SID. https://sid.ir/paper/241365/en

Vancouver: Copy

DEHDARI F., Mehrabi Koushki M., HAYATI J.. Identification of native fungi in contaminated soils of Marun oil field of Ahwaz. JOURNAL OF SOIL BIOLOGY[Internet]. 2019;7(2 ):149-165. Available from: https://sid.ir/paper/241365/en

IEEE: Copy

F. DEHDARI, M. Mehrabi Koushki, and J. HAYATI, “Identification of native fungi in contaminated soils of Marun oil field of Ahwaz,” JOURNAL OF SOIL BIOLOGY, vol. 7, no. 2 , pp. 149–165, 2019, [Online]. Available: https://sid.ir/paper/241365/en

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