GENETIC DIVERSITY AND SYMBIOTIC EFFECTIVENESS OF RHIZOBIA ISOLATED FROM ROOT NODULES OF MEDICAGO SATIVA L.

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SOLTANI TOOLAROOD A.A.; ALIKHANI H.A.; SALEHI JOZANI G.R.; ASADI RAHMANI H.; KHAVAZI K.; POURBABAEE A.A.

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Journal: Journal of Soil Biology Year:2013 | Volume:1 | Issue:1 Page(s): 1-12

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Title

GENETIC DIVERSITY AND SYMBIOTIC EFFECTIVENESS OF RHIZOBIA ISOLATED FROM ROOT NODULES OF MEDICAGO SATIVA L.

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Keywords

MEDICAGO SALIVA L.Q3

BIODIVERSITYQ3

PLANT INFECTIONQ2

SYMBIOTIC EFFECTIVENESSQ2

Abstract

The symbiotic nitrogen fixation resulting from the rhizobia-legume interaction can act as a sustainable source of nitrogen in many agricultural systems. Better N2 fixation can be achieved by selecting superior rhizobia from native populations. Genetic characterization and BIODIVERSITY of the native rhizobia population can be useful for the selection of inoculant strains. In this study, the PLANT INFECTION, SYMBIOTIC EFFECTIVENESS, and genetic diversity of 48 rhizobia isolated from root nodules of Medicago sativa L., cultivated in different sites of Hamedan Province, was studied by restriction fragment length polymorphism (RFLP) analysis of PCk-amplified intergenic spacer (ITS). Analysis of the 16S-23 S (ITS) fragment showed considerable diversity within these microsybionts. At the similarity of 70%, these rhizobia were clustered into 6 groups: I, II, III and IV. PLANT INFECTION test results showed that most strains formed nodules on the roots of host plant. No nodules were observed on the roots of plants inoculated with KH16, KH24, KH6, KH10, KH133, and KH193. According to the results of SYMBIOTIC EFFECTIVENESS test, strains were divided into four groups: non effective, relatively effective, effective, and highly effective strains.

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APA: Copy

SOLTANI TOOLAROOD, A.A., ALIKHANI, H.A., SALEHI JOZANI, G.R., ASADI RAHMANI, H., KHAVAZI, K., & POURBABAEE, A.A.. (2013). GENETIC DIVERSITY AND SYMBIOTIC EFFECTIVENESS OF RHIZOBIA ISOLATED FROM ROOT NODULES OF MEDICAGO SATIVA L.. JOURNAL OF SOIL BIOLOGY, 1(1), 1-12. SID. https://sid.ir/paper/241378/en

Vancouver: Copy

SOLTANI TOOLAROOD A.A., ALIKHANI H.A., SALEHI JOZANI G.R., ASADI RAHMANI H., KHAVAZI K., POURBABAEE A.A.. GENETIC DIVERSITY AND SYMBIOTIC EFFECTIVENESS OF RHIZOBIA ISOLATED FROM ROOT NODULES OF MEDICAGO SATIVA L.. JOURNAL OF SOIL BIOLOGY[Internet]. 2013;1(1):1-12. Available from: https://sid.ir/paper/241378/en

IEEE: Copy

A.A. SOLTANI TOOLAROOD, H.A. ALIKHANI, G.R. SALEHI JOZANI, H. ASADI RAHMANI, K. KHAVAZI, and A.A. POURBABAEE, “GENETIC DIVERSITY AND SYMBIOTIC EFFECTIVENESS OF RHIZOBIA ISOLATED FROM ROOT NODULES OF MEDICAGO SATIVA L.,” JOURNAL OF SOIL BIOLOGY, vol. 1, no. 1, pp. 1–12, 2013, [Online]. Available: https://sid.ir/paper/241378/en

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