POLYMORPHISMS IN EXON 17 OF DGAT1 GENE AND ITS ASSOCIATION WITH MILK PRODUCTION TRAITS IN MAHABADI GOAT BREED USING PCR-SSCP

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AZIZI ZAHRA; MORADI SHAHREBABAK HOSSEIN; MORADI SHAHREBABAK MOHAMMAD; ZALI ABOALFAZL

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Research On Animal Production Year:2014 | Volume:5 | Issue:9 Page(s): 108-117

Download Full-Text

Persian Verion

View:

650

Download:

Cites:

Information Journal Paper

Title

POLYMORPHISMS IN EXON 17 OF DGAT1 GENE AND ITS ASSOCIATION WITH MILK PRODUCTION TRAITS IN MAHABADI GOAT BREED USING PCR-SSCP

Author(s)

AZIZI ZAHRA | MORADI SHAHREBABAK HOSSEIN | MORADI SHAHREBABAK MOHAMMAD | ZALI ABOALFAZL | Issue Writer Certificate

Keywords

DGAT1Q2

POLYMORPHISMQ2

PCR-SSCPQ1

MAHABADI GOATQ2

MILK TRAITQ2

Abstract

Diacylglycerol transferase is a microsome enzyme that plays an important role in glycerol lipid metabolism. The acyl CoA-diacylglycerol- acyltransferase 1 (DGAT1) is considered to be the key enzyme in controlling the synthesis of triglycerides in adipocytes. This enzyme catalyzes the final step of triglyceride synthesis (transform triacylglycerol (DAG) into triacylglycerol (TAG). The present study has been designed to determine POLYMORPHISM in exon 17 of DGAT1 gene and their association with milk production traits in MAHABADI GOAT breed using PCR-SSCP. Blood sample were collected from 79 Mahabadi from research station of Animal Science Department at University of Tehran. DNA was extracted according to optimized salting out method. DNA quality and quantitative was determined with spectrophotometer and 1% agarose gel. A Fragment from exon 17 of DGAT1 gene was amplified using the polymerase chain reaction and SSCP technique was used to analyze POLYMORPHISM in this segment of DNA. PCR products were run in 12% polyacrylamide gel electrophoresis and were stained with silver nitrate. In overall, four SSCP patterns were observed with frequency of 0.25 (1), 0.24 (2), 0.06 (3) and 0.45 (4), respectively. The findings showed that DGAT1 gene had significant effect on milk production (P<0.01) and fat percent (P<0.05). Comparison of LS means showed that pattern 3 had the most effect on milk production (P<0.01) whereas pattern 1 was more effective on fat percentage (P<0.05). POLYMORPHISM of DGAT1 gene and its relationship with MILK TRAIT indicated that it probably can be useful in marker assisted selection and breeding programs.

Cites

No record.

References

No record.

Cite

APA: Copy

AZIZI, ZAHRA, MORADI SHAHREBABAK, HOSSEIN, MORADI SHAHREBABAK, MOHAMMAD, & ZALI, ABOALFAZL. (2014). POLYMORPHISMS IN EXON 17 OF DGAT1 GENE AND ITS ASSOCIATION WITH MILK PRODUCTION TRAITS IN MAHABADI GOAT BREED USING PCR-SSCP. RESEARCH ON ANIMAL PRODUCTION, 5(9), 108-117. SID. https://sid.ir/paper/241450/en

Vancouver: Copy

AZIZI ZAHRA, MORADI SHAHREBABAK HOSSEIN, MORADI SHAHREBABAK MOHAMMAD, ZALI ABOALFAZL. POLYMORPHISMS IN EXON 17 OF DGAT1 GENE AND ITS ASSOCIATION WITH MILK PRODUCTION TRAITS IN MAHABADI GOAT BREED USING PCR-SSCP. RESEARCH ON ANIMAL PRODUCTION[Internet]. 2014;5(9):108-117. Available from: https://sid.ir/paper/241450/en

IEEE: Copy

ZAHRA AZIZI, HOSSEIN MORADI SHAHREBABAK, MOHAMMAD MORADI SHAHREBABAK, and ABOALFAZL ZALI, “POLYMORPHISMS IN EXON 17 OF DGAT1 GENE AND ITS ASSOCIATION WITH MILK PRODUCTION TRAITS IN MAHABADI GOAT BREED USING PCR-SSCP,” RESEARCH ON ANIMAL PRODUCTION, vol. 5, no. 9, pp. 108–117, 2014, [Online]. Available: https://sid.ir/paper/241450/en

Related Journal Papers