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Information Journal Paper

Title

EVALUATION OF DNA VACCINE ENCODING HPV-16 E7 ON LYMPHOCYTE PROLIFERATION INDUCTION IN HUMAN PAPILLOMA VIRUS-ASSOCIATED TUMOR ANIMAL MODELS

Pages

  14-24

Abstract

 Background & Objective: Human papillomavirus (HPV) oncoproteins, including E6 and E7 are constitutively expressed in cervical cancer cells. These proteins are ideal targets to be used for developing therapeutic vaccines against existing HPV-associated carcinomas. The aim of this study was to measure the proliferation response rate of splenic lymphocytes derived from E7- HPV16 encoding plasmid injection on the tumor mouse model of papillomavirus.Method: C57BL/6 mice were inoculated subcutaneous with 5× 10 TC-1 cells in three times with two weeks intervals and then immunized with HPV-16 E7 DNA VACCINE. The proliferation response of splenic cells was measured by MTT assay. IL12 cytokine was measured by ELISA assay and the mass of tumor was calculated with caliper for six weeks.Results: Following the application of DNA VACCINEs containing E7 therapeutic gene, the proliferative response of splenic cells was provoked significanltly higher than the stimulation in control group (P<0.05). Moreover, the secretion of IL12 was significantly increased in vaccinated mice tumor tissue (P<0.05). The growth of tumor in vaccinated group was markedly decreased in comparison to PBS and pcDNA3 groups (P<0.05).Conclusion: Our findings revealed that the application of DNA VACCINE containing E7 gene in a tumor mouse model may induce anti-tumor cellular immune responses.

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    APA: Copy

    BAGHBAN RAHIMI, SANAZ, SOLEIMANJAHI, HOORIEH, MOHEBBI, ALIREZA, EBRAHIMZADEH, MIR SAEID, ALIZADEH, LEYLA, & GHAEMI, AMIR. (2017). EVALUATION OF DNA VACCINE ENCODING HPV-16 E7 ON LYMPHOCYTE PROLIFERATION INDUCTION IN HUMAN PAPILLOMA VIRUS-ASSOCIATED TUMOR ANIMAL MODELS. JORJANI BIOMEDICINE JOURNAL, 5(1), 14-24. SID. https://sid.ir/paper/241559/en

    Vancouver: Copy

    BAGHBAN RAHIMI SANAZ, SOLEIMANJAHI HOORIEH, MOHEBBI ALIREZA, EBRAHIMZADEH MIR SAEID, ALIZADEH LEYLA, GHAEMI AMIR. EVALUATION OF DNA VACCINE ENCODING HPV-16 E7 ON LYMPHOCYTE PROLIFERATION INDUCTION IN HUMAN PAPILLOMA VIRUS-ASSOCIATED TUMOR ANIMAL MODELS. JORJANI BIOMEDICINE JOURNAL[Internet]. 2017;5(1):14-24. Available from: https://sid.ir/paper/241559/en

    IEEE: Copy

    SANAZ BAGHBAN RAHIMI, HOORIEH SOLEIMANJAHI, ALIREZA MOHEBBI, MIR SAEID EBRAHIMZADEH, LEYLA ALIZADEH, and AMIR GHAEMI, “EVALUATION OF DNA VACCINE ENCODING HPV-16 E7 ON LYMPHOCYTE PROLIFERATION INDUCTION IN HUMAN PAPILLOMA VIRUS-ASSOCIATED TUMOR ANIMAL MODELS,” JORJANI BIOMEDICINE JOURNAL, vol. 5, no. 1, pp. 14–24, 2017, [Online]. Available: https://sid.ir/paper/241559/en

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