POLYMORPHISM INVESTIGATION OF SLC24A5 GENE IN RAINBOW TROUT (ONCORHYNCHUS MYKISS)

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KARIMKHANI M.; YOUSEFI SIAHKALROODI S.; ASGARI JAFARABADI GH.

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Journal: Journal of Aquaculture Development Year:2016 | Volume:9 | Issue:4 Page(s): 43-51

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Title

POLYMORPHISM INVESTIGATION OF SLC24A5 GENE IN RAINBOW TROUT (ONCORHYNCHUS MYKISS)

Author(s)

KARIMKHANI M. | YOUSEFI SIAHKALROODI S. | ASGARI JAFARABADI GH. | Issue Writer Certificate

Keywords

POLYMORPHISM

RAINBOW TROUT (ONCORHYNCHUS MYKISS)

SLC24A5

PCR-RFLP

Abstract

In this survey POLYMORPHISM of SLC24A5 gene of Rainbow trout (Oncorhynchus mykiss) was studied. Samples were prepared from 100 Rainbow trout muscles. After DNA extraction, part of gene from POLYMORPHISM area with 1000 bp amplified by PCR then cut with Taq1 inhibiting enzymeand electrophoresed on agarose gel 1.5%. Results shows that M and N alleles are in this area of gene, and the frequency of them is 0.72 and 0.28 in population respectively. MM and NN genotypes were distinguished which frequency of them were estimated 0.72 and 0.28 in whole population respectively. Chi-Square test for this part of SLC24A5 gene was not similar with Hardy–Weinberg equilibrium. It shows non-random mating, small population and increase in inbreeding and homozygotes increase in the population of this study. Furthermore Rainbow trout is so important in aquaculture industry in Iran the notability of this issue is significant because SLC24A5 gene is highly inheritable and selection of that can improve the color of meat.

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APA: Copy

KARIMKHANI, M., YOUSEFI SIAHKALROODI, S., & ASGARI JAFARABADI, GH.. (2016). POLYMORPHISM INVESTIGATION OF SLC24A5 GENE IN RAINBOW TROUT (ONCORHYNCHUS MYKISS). JOURNAL OF AQUACULTURE DEVELOPMENT, 9(4), 43-51. SID. https://sid.ir/paper/242014/en

Vancouver: Copy

KARIMKHANI M., YOUSEFI SIAHKALROODI S., ASGARI JAFARABADI GH.. POLYMORPHISM INVESTIGATION OF SLC24A5 GENE IN RAINBOW TROUT (ONCORHYNCHUS MYKISS). JOURNAL OF AQUACULTURE DEVELOPMENT[Internet]. 2016;9(4):43-51. Available from: https://sid.ir/paper/242014/en

IEEE: Copy

M. KARIMKHANI, S. YOUSEFI SIAHKALROODI, and GH. ASGARI JAFARABADI, “POLYMORPHISM INVESTIGATION OF SLC24A5 GENE IN RAINBOW TROUT (ONCORHYNCHUS MYKISS),” JOURNAL OF AQUACULTURE DEVELOPMENT, vol. 9, no. 4, pp. 43–51, 2016, [Online]. Available: https://sid.ir/paper/242014/en

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