ISOLATION AND IDENTIFICATION OF YERSINIA RUCKERI BY API 20E BIOCHEMICAL METHOD AND MOLECULAR CONFIRMATION OF ISOLATION BY 16SR DNA PCR SEQUENCING IN RAINBOW TROUT (ONCORHYNCHUS MYKISS), IN NORTHERN IRAN

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JAFARPUR M.; MOSAZADEH SARGHEIN M.; NAZEMI A.; ARABI SH.; HESHMATIPOOR Z.

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Journal: Journal of Aquaculture Development Year:2013 | Volume:7 | Issue:2 Page(s): 9-15

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Title

ISOLATION AND IDENTIFICATION OF YERSINIA RUCKERI BY API 20E BIOCHEMICAL METHOD AND MOLECULAR CONFIRMATION OF ISOLATION BY 16SR DNA PCR SEQUENCING IN RAINBOW TROUT (ONCORHYNCHUS MYKISS), IN NORTHERN IRAN

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Keywords

YERSINIA RUCKERIQ2

ERMQ2

PCRQ2

SEQUENCINGQ2

API20EGALLERYQ2

Abstract

YERSINIA RUCKERIis the etiological agent of enteric red mouth disease (ERM) or yersiniosis, a general septicemia affecting mainly Salmonids. This disease is common word wide and produced considerable economic losses in the fish farming industry. Therefore current identification of pathogen from fish is essential for the effective fish disease control. An epidemic of disease in rain bow trout was occurred in Tonekabon city in Iran. In this study as well as biochemical detection we confirmed that laboratory culture based method by alignment analysis from sequences that outcome of PCR that used by universal primers. In contrast to biochemical same study in Romania that had detected Yersiniaby using the API 20E gallery, numerical values corresponding to the each group have been changed from 5.104, 100 at Romania to 5, 105, 100. in Iran. this difference due to positive VP test in our study and negative VP test was carried out in Romania The high throughput and excision detection rDNA PCR- SEQUENCING used by universal primers rather than conventional method in this study provide a powerful addition tool to conventional methods for more accurate detection and monitoring of YERSINIA RUCKERI. This study reports the first biochemical identification of YERSINIA RUCKERI by API Gallery and its confirmation by rDNA- PCR-Sequencing at the country of Iran.

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APA: Copy

JAFARPUR, M., MOSAZADEH SARGHEIN, M., NAZEMI, A., ARABI, SH., & HESHMATIPOOR, Z.. (2013). ISOLATION AND IDENTIFICATION OF YERSINIA RUCKERI BY API 20E BIOCHEMICAL METHOD AND MOLECULAR CONFIRMATION OF ISOLATION BY 16SR DNA PCR SEQUENCING IN RAINBOW TROUT (ONCORHYNCHUS MYKISS), IN NORTHERN IRAN. JOURNAL OF AQUACULTURE DEVELOPMENT, 7(2), 9-15. SID. https://sid.ir/paper/242043/en

Vancouver: Copy

JAFARPUR M., MOSAZADEH SARGHEIN M., NAZEMI A., ARABI SH., HESHMATIPOOR Z.. ISOLATION AND IDENTIFICATION OF YERSINIA RUCKERI BY API 20E BIOCHEMICAL METHOD AND MOLECULAR CONFIRMATION OF ISOLATION BY 16SR DNA PCR SEQUENCING IN RAINBOW TROUT (ONCORHYNCHUS MYKISS), IN NORTHERN IRAN. JOURNAL OF AQUACULTURE DEVELOPMENT[Internet]. 2013;7(2):9-15. Available from: https://sid.ir/paper/242043/en

IEEE: Copy

M. JAFARPUR, M. MOSAZADEH SARGHEIN, A. NAZEMI, SH. ARABI, and Z. HESHMATIPOOR, “ISOLATION AND IDENTIFICATION OF YERSINIA RUCKERI BY API 20E BIOCHEMICAL METHOD AND MOLECULAR CONFIRMATION OF ISOLATION BY 16SR DNA PCR SEQUENCING IN RAINBOW TROUT (ONCORHYNCHUS MYKISS), IN NORTHERN IRAN,” JOURNAL OF AQUACULTURE DEVELOPMENT, vol. 7, no. 2, pp. 9–15, 2013, [Online]. Available: https://sid.ir/paper/242043/en

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