ASSESSING DIFFERENT METHODS OF DNA EXTRACTION FROM THE GOLDEN RAINBOW TROUT FARMED (GOLDEN RAINBOW TROUT: ONCORHYNCHUS MYKISS) IN ORDER TO STUDY THE MOLECULAR

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AKBARNEJAD B.; YOUSEFI SIAHKALRODI S.; ZARE KARIZI SH.

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Journal: Journal of Aquaculture Development Year:2018 | Volume:12 | Issue:2 Page(s): 1-11

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Title

ASSESSING DIFFERENT METHODS OF DNA EXTRACTION FROM THE GOLDEN RAINBOW TROUT FARMED (GOLDEN RAINBOW TROUT: ONCORHYNCHUS MYKISS) IN ORDER TO STUDY THE MOLECULAR

Author(s)

AKBARNEJAD B. | YOUSEFI SIAHKALRODI S. | ZARE KARIZI SH. | Issue Writer Certificate

Keywords

GOLDEN RAINBOW TROUT FARMEDQ1

DNA EXTRACTIONQ1

COMPANY EXTRACTION KIT GENE ALLQ1

PCRQ2

Abstract

DNA extraction is a routine step in many biological studies and variety of methods have been established to isolate DNA molecules from biological materials. most methods share three aims: 1- the comprehensive lysis of cells and extraction of intracellular nucleic acids into aqueous solution, 2- the removal of non-nucleic acid organic and inorganic molecules from resultant aqueous extracts and 3-the minimization of nucleic acid losses throughout this purification process. Rainbow trout fish, including salmonid species have been domesticated and today the most important cold-water fish species in the country. To accomplish this study 4 Extraction 1. Extraction Kit Company Gena All 2-Trizol Reagent 3- traditional method of heating and Proteinase K 4- phenol chloroform were studied and It was found that the The best extraction method in terms of quality and the quantity of DNA extracted by 1 percent agarose gel and Nanodrop and Also in terms of the effectiveness of PCR for DNA EXTRACTION kit method is the enterprise Gene All Country South Korea.

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APA: Copy

AKBARNEJAD, B., YOUSEFI SIAHKALRODI, S., & ZARE KARIZI, SH.. (2018). ASSESSING DIFFERENT METHODS OF DNA EXTRACTION FROM THE GOLDEN RAINBOW TROUT FARMED (GOLDEN RAINBOW TROUT: ONCORHYNCHUS MYKISS) IN ORDER TO STUDY THE MOLECULAR. JOURNAL OF AQUACULTURE DEVELOPMENT, 12(2 ), 1-11. SID. https://sid.ir/paper/242122/en

Vancouver: Copy

AKBARNEJAD B., YOUSEFI SIAHKALRODI S., ZARE KARIZI SH.. ASSESSING DIFFERENT METHODS OF DNA EXTRACTION FROM THE GOLDEN RAINBOW TROUT FARMED (GOLDEN RAINBOW TROUT: ONCORHYNCHUS MYKISS) IN ORDER TO STUDY THE MOLECULAR. JOURNAL OF AQUACULTURE DEVELOPMENT[Internet]. 2018;12(2 ):1-11. Available from: https://sid.ir/paper/242122/en

IEEE: Copy

B. AKBARNEJAD, S. YOUSEFI SIAHKALRODI, and SH. ZARE KARIZI, “ASSESSING DIFFERENT METHODS OF DNA EXTRACTION FROM THE GOLDEN RAINBOW TROUT FARMED (GOLDEN RAINBOW TROUT: ONCORHYNCHUS MYKISS) IN ORDER TO STUDY THE MOLECULAR,” JOURNAL OF AQUACULTURE DEVELOPMENT, vol. 12, no. 2 , pp. 1–11, 2018, [Online]. Available: https://sid.ir/paper/242122/en

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