IN VITRO FLOWER BUD FORMATION, PLANT REGENERATION AND MORPHOGENETIC STUDIES IN LOCAL SCENTED CULTIVAR OF ROSA INDICA

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BIMAL RAAGEEVA; KIRAN NITI

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Journal: JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS) Year:2014 | Volume:4 | Issue:1 Page(s): 9-18

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Title

IN VITRO FLOWER BUD FORMATION, PLANT REGENERATION AND MORPHOGENETIC STUDIES IN LOCAL SCENTED CULTIVAR OF ROSA INDICA

Author(s)

BIMAL RAAGEEVA | KIRAN NITI | Issue Writer Certificate

Keywords

MULTIPLE SHOOTSQ2

‘QUIC ROOT’Q2

ROSEQ2

ROSA INDICAQ2

TISSUE CULTUREQ3

Abstract

In vitro propagation of ROSE (Rosa indica) as well as ROSE breeding is a valuable economic enterprise and has received highest importance globally including Indian subcontinent. The development of new cultivars for commerce is faced with new challenges. In vitro micropropagation protocols of varieties of ROSEs have been established but very few reports on the clonal propagation of local cultivars of Indian scented ROSE has been made. In the present paper we report the dedifferentiation and redifferentiation of stem nodal, leaf disc, petals well as ovary explants cultured on MS medium supplemented with various growth adjuvants such as coconut water (CW 5%-20% v/v), auxins (IAA, NAA, 2, 4-D and IBA) and cytokinins (BAP and Kn) in varying concentrations. Vigorous callus formation was observed showing embryogenic clusters in both the nodal and leaf disc explants on MS medium supplemented with CW (10% v/v) +2, 4- D (11.3 mM) +BAP (3.55 mM). The proliferation of 4-7 MULTIPLE SHOOTS (mean number of shoots 5±1.24) as well as in vitro flower bud formation on in vitro regenerated ROSE plants from nodal explants cultured on MS medium supplemented with CW+2, 4-D+BAP have been observed. The regenerated shoots treated with ‘QUIC ROOT’ (commercially available rooting chemical) produced well developed root system.

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APA: Copy

BIMAL, RAAGEEVA, & KIRAN, NITI. (2014). IN VITRO FLOWER BUD FORMATION, PLANT REGENERATION AND MORPHOGENETIC STUDIES IN LOCAL SCENTED CULTIVAR OF ROSA INDICA. JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS), 4(1), 9-18. SID. https://sid.ir/paper/242607/en

Vancouver: Copy

BIMAL RAAGEEVA, KIRAN NITI. IN VITRO FLOWER BUD FORMATION, PLANT REGENERATION AND MORPHOGENETIC STUDIES IN LOCAL SCENTED CULTIVAR OF ROSA INDICA. JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS)[Internet]. 2014;4(1):9-18. Available from: https://sid.ir/paper/242607/en

IEEE: Copy

RAAGEEVA BIMAL, and NITI KIRAN, “IN VITRO FLOWER BUD FORMATION, PLANT REGENERATION AND MORPHOGENETIC STUDIES IN LOCAL SCENTED CULTIVAR OF ROSA INDICA,” JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS), vol. 4, no. 1, pp. 9–18, 2014, [Online]. Available: https://sid.ir/paper/242607/en

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