THE OPTIMIZATION AND COMPARISON OF DIFFERENT EXTRACTION APPROACHES (ULTRASOUND AND MACERATION) ON CHEMICAL COMPOSITION OF ELAEAGNUS ANGUSTIFOLIAL L. EXTRACT

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SARDARODIYAN MARYAM; MEHRABAN SANG ATASH MASOME; ARIANFAR AKRAM

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Journal: Ecophytochemistry journal of Medicinal Plants Year:2016 | Volume:4 | Issue:3 (15) Page(s): 79-94

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Title

THE OPTIMIZATION AND COMPARISON OF DIFFERENT EXTRACTION APPROACHES (ULTRASOUND AND MACERATION) ON CHEMICAL COMPOSITION OF ELAEAGNUS ANGUSTIFOLIAL L. EXTRACT

Author(s)

SARDARODIYAN MARYAM | MEHRABAN SANG ATASH MASOME | ARIANFAR AKRAM | Issue Writer Certificate

Keywords

OPTIMIZATIONQ2

EXTRACTION METHODQ2

ULTRASOUNDQ2

MACERATIONQ2

ELAEAGNUS ANGUSTIFOLIA LQ2

Abstract

Elaeagnus angustifolia L. is regarded as one of the most important medicinal and nutritious plants since it contains flavonoid, sitosterols, cardiac glycosides and terpenoids compounds. The purpose of this study was to investigating the effects of extraction conditions on phenol and flavonoid content from methanolic extracts of Elaeagnus angustifolia (peel, pulp and seed) through ULTRASOUND and MACERATION methods. The response surface methodology (RSM) was used to optimize the extraction conditions for highest extraction rates of phenolic and flavonoid compounds. Box Behnken design was employed to investigate the effects of the three independent variables including solvent to sample ratio (20-50 ml/g), temperature (30-60oC) and extraction time (5-35 min) on the dependent variables (extraction yield, phenolic and flavonoid contents). The total phenolic content was measured using the Folin-Ciocalteu’s reagent. The total flavonoid content was assessed by the colorimetric method based on Aluminum Chloride which underwent some changes. The variance coefficients indicated that the effect of ULTRASOUND temperature on the extraction yield was more influential than the other ones (p<0.05). The optimum extraction condition was solvent to sample ratio (31.79 ml/g), temperature (52.87oC (and extraction time (25.44 min (. The optimum conditions, extraction yield, phenolic and flavonoid contents were 21.58 mg/g, 106.31 (mgGAEg-1) and 32.98 (mgQUEg-1), respectively. Considering the comparison between the two EXTRACTION METHODs, it was shown that extraction efficiency of phenolic and flavonoid compounds through ULTRASOUND method is more than MACERATION method. Thus, the use of ULTRASOUND-assisted extraction is recommended to extract phenolic compounds from the fruits of Elaeagnus angustifolia.

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APA: Copy

SARDARODIYAN, MARYAM, MEHRABAN SANG ATASH, MASOME, & ARIANFAR, AKRAM. (2016). THE OPTIMIZATION AND COMPARISON OF DIFFERENT EXTRACTION APPROACHES (ULTRASOUND AND MACERATION) ON CHEMICAL COMPOSITION OF ELAEAGNUS ANGUSTIFOLIAL L. EXTRACT. ECO-PHYTOCHEMICAL JOURNAL OF MEDICAL PLANTS, 4(3 (15)), 79-94. SID. https://sid.ir/paper/247795/en

Vancouver: Copy

SARDARODIYAN MARYAM, MEHRABAN SANG ATASH MASOME, ARIANFAR AKRAM. THE OPTIMIZATION AND COMPARISON OF DIFFERENT EXTRACTION APPROACHES (ULTRASOUND AND MACERATION) ON CHEMICAL COMPOSITION OF ELAEAGNUS ANGUSTIFOLIAL L. EXTRACT. ECO-PHYTOCHEMICAL JOURNAL OF MEDICAL PLANTS[Internet]. 2016;4(3 (15)):79-94. Available from: https://sid.ir/paper/247795/en

IEEE: Copy

MARYAM SARDARODIYAN, MASOME MEHRABAN SANG ATASH, and AKRAM ARIANFAR, “THE OPTIMIZATION AND COMPARISON OF DIFFERENT EXTRACTION APPROACHES (ULTRASOUND AND MACERATION) ON CHEMICAL COMPOSITION OF ELAEAGNUS ANGUSTIFOLIAL L. EXTRACT,” ECO-PHYTOCHEMICAL JOURNAL OF MEDICAL PLANTS, vol. 4, no. 3 (15), pp. 79–94, 2016, [Online]. Available: https://sid.ir/paper/247795/en

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