INVESTIGATION OF THE RATIO OF X AND Y CHROMOSOMES POPULATION IN HOLSTEIN BULLS’ EJACULATION AND THE ROLE OF BLOOD TESTOSTERONE CONCENTRATION ON POPULATION RATIO

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KHOLGHI M.; HEYDARI F.; ROSTAMZADEH J.; RAZMKABIR M.

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Journal: Journal of Molecular and Cellular Researches Year:2016 | Volume:29 | Issue:1 Page(s): 72-79

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Title

INVESTIGATION OF THE RATIO OF X AND Y CHROMOSOMES POPULATION IN HOLSTEIN BULLS’ EJACULATION AND THE ROLE OF BLOOD TESTOSTERONE CONCENTRATION ON POPULATION RATIO

Author(s)

KHOLGHI M. | HEYDARI F. | ROSTAMZADEH J. | RAZMKABIR M. | Issue Writer Certificate

Keywords

SRY GENEQ1

PLP GENEQ1

SEX RATIOQ2

TESTOSTERONE CONCENTRATIONQ1

REAL TIME-PCRQ1

Abstract

The ability and possibility of calf sex determination is very important in animal husbandry industry and is one of old aims in dairy and beef cattle industry. Due to being heterogamete, bull has a greater contribution in sexual ratio than cattle. Specification of sexual ratio in spermatozoids is done with X and Y chromosomes ratio. This study was conducted in order to determine the effect of blood TESTOSTERONE CONCENTRATION on change in X and Y sexual chromosomes’ ratio of spermatozoids in Holstein bulls using Real-Time PCR technique. In this study, blood and sperm samples were collected from 26 Holstein bulls at breeding center in Abbas Abad station. After DNA extraction from sperms by Salting out method, in order to proliferation of 90, 89 and 79 base pair pieces for PLP, SRY and PAR genes respectively, Real-Time PCR was done using specific primer and sexual ratio was calculated. The results of statistical analysis showed that the least square mean of X and Y chromosomes was 1.23±0.15 and 0.71±0.02 respectively and had a significant difference. Also, the correlation of SRY GENE with blood TESTOSTERONE CONCENTRATION was estimated 0.38 that these results reveal that with an increase in the level of testosterone, the relative amount of spermatozoids carrying SRY GENE will increase significantly. Additionally, correlation between PLP GENE and blood testosterone was estimated 0.67, so with an increase in TESTOSTERONE CONCENTRATION, the relative amount of spermatozoids carrying PLP GENE decrease significantly.

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APA: Copy

KHOLGHI, M., HEYDARI, F., ROSTAMZADEH, J., & RAZMKABIR, M.. (2016). INVESTIGATION OF THE RATIO OF X AND Y CHROMOSOMES POPULATION IN HOLSTEIN BULLS’ EJACULATION AND THE ROLE OF BLOOD TESTOSTERONE CONCENTRATION ON POPULATION RATIO. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 29(1 ), 72-79. SID. https://sid.ir/paper/248432/en

Vancouver: Copy

KHOLGHI M., HEYDARI F., ROSTAMZADEH J., RAZMKABIR M.. INVESTIGATION OF THE RATIO OF X AND Y CHROMOSOMES POPULATION IN HOLSTEIN BULLS’ EJACULATION AND THE ROLE OF BLOOD TESTOSTERONE CONCENTRATION ON POPULATION RATIO. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2016;29(1 ):72-79. Available from: https://sid.ir/paper/248432/en

IEEE: Copy

M. KHOLGHI, F. HEYDARI, J. ROSTAMZADEH, and M. RAZMKABIR, “INVESTIGATION OF THE RATIO OF X AND Y CHROMOSOMES POPULATION IN HOLSTEIN BULLS’ EJACULATION AND THE ROLE OF BLOOD TESTOSTERONE CONCENTRATION ON POPULATION RATIO,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 29, no. 1 , pp. 72–79, 2016, [Online]. Available: https://sid.ir/paper/248432/en

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