IN VITRO MICROPROPAGATION OF CATHARANTHUS ROSEUS (LINN.) G. DON VIA SHOOT MULTIPLICATION

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AHMADI J.; MOHAMMADI R.; GAROUSI GH.A.

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Journal: Journal of Molecular and Cellular Researches Year:2014 | Volume:27 | Issue:1 Page(s): 14-25

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Title

IN VITRO MICROPROPAGATION OF CATHARANTHUS ROSEUS (LINN.) G. DON VIA SHOOT MULTIPLICATION

Author(s)

AHMADI J. | MOHAMMADI R. | GAROUSI GH.A. | Issue Writer Certificate

Keywords

CATHARANTHUS ROSEUSQ2

SHOOT INDUCTION. ROOTINGQ2

MICROPROPAGATIONQ2

Abstract

Periwinkle (CATHARANTHUS ROSEUS (Linn.) G. Don) is known as an important medicinal plant because it contains vincristine and vinblastine alkaloids. Therefore development of micro-propagation in vitro culture has high importance. The present study describes two shoot induction and one root induction optimization experiments. The experimental designs were 3´5 factorial (BAP´NAA and KN´NAA) for shoot induction trails and 3´5 factorial (IBA´NAA) for root induction trail based on completely randomized design with four replications. The best proliferation of shoot induction percent and shoot per explants was shown on the medium supplemented with high KN and NAA, respectively. In overall, KN (0.1 mg/l) + NAA (1 mg/l) combination proved to be optimal for the production of maximum number of shoots. Mean comparison showed that the highest multiplication rate was obtained with 0.1 mg/l of 6-benzylaminopurine (87%) and with 0.1 mg/l 6-benzylaminopurine +2 mg/l naphthalene acetic acid (100%). The best condition for rooting (root number and length) was 0.4 mg/l IBA +4 mg/l naphthalene acetic acid and the maximum root length was observed on medium supplemented with higher NAA concentration.

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