ANTIMICROBIAL EFFECTS OF TRICHODERMA HARZIANUM CRUDE EXTRACT AND ITS EFFICACY IN BIOCONTROL

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KAZEMZADEH S.; FARROKHI N.; AMINZADEH S.; ALAVI S.M.; SARPELE A.; MAMARABADI M.

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Journal: Journal of Molecular and Cellular Researches Year:2015 | Volume:28 | Issue:1 Page(s): 107-114

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Title

ANTIMICROBIAL EFFECTS OF TRICHODERMA HARZIANUM CRUDE EXTRACT AND ITS EFFICACY IN BIOCONTROL

Author(s)

Keywords

TRICHODERMAQ2

ANTIBACTERAIL PROTEINQ2

ANTAGONISTQ2

PATHOGENQ2

Abstract

TRICHODERMA harzianum crude extract was prepared and its controlling effects on a wide range of Gram positive and negative bacteria and a few fungal species were evaluated via disk assay techniques. The extract was able to control PATHOGENic bacteria including Escherichia coli, Bacillus subtilis, Pseudomonas fluorescens, Salmonella typhi, Staphylococcus aureus, two Xanthomonas citri pathovars (NIGEB-88 and NIGEB- 9322), and also two fungal PATHOGENs: Macrophomina phaseolina and Rhizoctonia solani. However, it failed to control a pectobacter, Erwinia amylovora. The biocontrol efficacy of crude extract was lost once treated with trypsin and reduced in the presence of SDS, indicating the protein nature of the effective materials in the fungal extract. The antimicrobial protein was resisted 100oC for 30 min and was active in a wide range of pH (4-9). Our data is indicative of an efficient isolation of novel fungal thermostable antimicrobial proteins with the potential to be used as a biocontrol agent in the future.

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APA: Copy

KAZEMZADEH, S., FARROKHI, N., AMINZADEH, S., ALAVI, S.M., SARPELE, A., & MAMARABADI, M.. (2015). ANTIMICROBIAL EFFECTS OF TRICHODERMA HARZIANUM CRUDE EXTRACT AND ITS EFFICACY IN BIOCONTROL. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(1), 107-114. SID. https://sid.ir/paper/248491/en

Vancouver: Copy

KAZEMZADEH S., FARROKHI N., AMINZADEH S., ALAVI S.M., SARPELE A., MAMARABADI M.. ANTIMICROBIAL EFFECTS OF TRICHODERMA HARZIANUM CRUDE EXTRACT AND ITS EFFICACY IN BIOCONTROL. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(1):107-114. Available from: https://sid.ir/paper/248491/en

IEEE: Copy

S. KAZEMZADEH, N. FARROKHI, S. AMINZADEH, S.M. ALAVI, A. SARPELE, and M. MAMARABADI, “ANTIMICROBIAL EFFECTS OF TRICHODERMA HARZIANUM CRUDE EXTRACT AND ITS EFFICACY IN BIOCONTROL,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 1, pp. 107–114, 2015, [Online]. Available: https://sid.ir/paper/248491/en

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