THE EFFECT OF CALCIUM ON THE ACTIVATION OF THERMOLYSIN BY SALT

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VARASTEH A.; ASGHARI S.M.; TAGHDIR M.; AGHAMAALI M.R.; PAZHANG M.; MEHRNEJAD F.

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Journal: Journal of Molecular and Cellular Researches Year:2017 | Volume:30 | Issue:1 Page(s): 100-105

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Title

THE EFFECT OF CALCIUM ON THE ACTIVATION OF THERMOLYSIN BY SALT

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Keywords

THERMOLYSINQ2

ACTIVITYQ2

SODIUM CHLORIDEQ2

CALCIUMQ2

Abstract

THERMOLYSIN, obtained frombacillus thermoproteolyticus, is a thermophilic protease having application in peptide synthesis. Previous studies have revealed that salt activates the enzyme. Thermal stability of TLN is critically dependent on four CALCIUM ions bound in N- and C-terminal domains. The aim of present study is unraveling the role of CALCIUM ions in the salt activation of enzyme. In the presence of NaCl2 M, measurement of the ACTIVITY of enzyme in the absence and presence of CaCl2 10 mM using FAGLA as substrate was investigated. In addition, Far-UV circular dichroism and fluorescence spectroscopies were used to understand the CALCIUM dependent changes in the enzyme structure. Results revealed the activation of enzyme using FAGLA and in the presence of CaCl2 10 mM increased 2.5 times. Far-UV circular dichroism spectra suggest that the overall structure of THERMOLYSIN has not changed during salt and CALCIUM activation. However, fluorescence studies revealed that the active site has changed under CALCIUM-dependent salt activation. According to the results of the present study, the binding of CALCIUM ions might influence the activation of THERMOLYSIN by salt.

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APA: Copy

VARASTEH, A., ASGHARI, S.M., TAGHDIR, M., AGHAMAALI, M.R., PAZHANG, M., & MEHRNEJAD, F.. (2017). THE EFFECT OF CALCIUM ON THE ACTIVATION OF THERMOLYSIN BY SALT. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 30(1 ), 100-105. SID. https://sid.ir/paper/248500/en

Vancouver: Copy

VARASTEH A., ASGHARI S.M., TAGHDIR M., AGHAMAALI M.R., PAZHANG M., MEHRNEJAD F.. THE EFFECT OF CALCIUM ON THE ACTIVATION OF THERMOLYSIN BY SALT. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2017;30(1 ):100-105. Available from: https://sid.ir/paper/248500/en

IEEE: Copy

A. VARASTEH, S.M. ASGHARI, M. TAGHDIR, M.R. AGHAMAALI, M. PAZHANG, and F. MEHRNEJAD, “THE EFFECT OF CALCIUM ON THE ACTIVATION OF THERMOLYSIN BY SALT,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 30, no. 1 , pp. 100–105, 2017, [Online]. Available: https://sid.ir/paper/248500/en

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