LEAF EXPLANT BROWNING OF FOUR SPECIES OF SOLANACEAE FAMILY AFTER INDUCTION BY AGROBACTERIUM RHIZOGENES SPECIES

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SHAKERAN Z.; KEYHANFAR M.

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Journal: Journal of Molecular and Cellular Researches Year:2017 | Volume:30 | Issue:3 Page(s): 368-378

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Title

LEAF EXPLANT BROWNING OF FOUR SPECIES OF SOLANACEAE FAMILY AFTER INDUCTION BY AGROBACTERIUM RHIZOGENES SPECIES

Author(s)

SHAKERAN Z. | KEYHANFAR M. | Issue Writer Certificate

Keywords

LEAF EXPLANTQ1

AGROBACTERIUM RHIZOGENESQ1

BROWNINGQ2

HAIRY ROOTQ1

SOLANACEAEQ1

Abstract

The HAIRY ROOT formation is performed by transferring of special genes of AGROBACTERIUM RHIZOGENESplasmid's to the explants. These roots are used to increase of valuable pharmaceutical secondary metabolites. One of the most important factors that inhibits the HAIRY ROOT formation after induction of explants byA. rhizogenes is BROWNING. The cause of BROWNING in LEAF EXPLANTs is probably release of phenolic compounds from the wound site or defense mechanisms performed in plant cells against infection byA. rhizogenes. In this study, the root explants of four species of SOLANACEAE family includingAtropa belladonna, Hyoscyamus niger, Datura stramonium and Datura metelwere induced by different strains of A. rhizogenes (AR318, AR15834, AR9543, A7, AR9402, and A4) and the percent of BROWNING evaluated in these explants. For the results, the minimum percent (2% and 6%) of BROWNING observed in LEAF EXPLANTs that induced by strains of A4 and AR15834 respectively. Hence, the use of these strains is appropriate for induction of HAIRY ROOTs in thesolanaceae family.

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APA: Copy

SHAKERAN, Z., & KEYHANFAR, M.. (2017). LEAF EXPLANT BROWNING OF FOUR SPECIES OF SOLANACEAE FAMILY AFTER INDUCTION BY AGROBACTERIUM RHIZOGENES SPECIES. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 30(3 ), 368-378. SID. https://sid.ir/paper/248538/en

Vancouver: Copy

SHAKERAN Z., KEYHANFAR M.. LEAF EXPLANT BROWNING OF FOUR SPECIES OF SOLANACEAE FAMILY AFTER INDUCTION BY AGROBACTERIUM RHIZOGENES SPECIES. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2017;30(3 ):368-378. Available from: https://sid.ir/paper/248538/en

IEEE: Copy

Z. SHAKERAN, and M. KEYHANFAR, “LEAF EXPLANT BROWNING OF FOUR SPECIES OF SOLANACEAE FAMILY AFTER INDUCTION BY AGROBACTERIUM RHIZOGENES SPECIES,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 30, no. 3 , pp. 368–378, 2017, [Online]. Available: https://sid.ir/paper/248538/en

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