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Information Journal Paper

Title

EVALUATION OF ANTAGONISTIC EFFECT AND GENETIC DIVERSITY OF STREPTOMYCES STRAINS ISOLATED FROM SOILS OF THE KERMAN PROVINCE AS BIOLOGICAL CONTROL OF SCLEROTINIA SCLEROTIORUM

Pages

  357-367

Abstract

 At the present research, 30 isolates of Actinomycetes have been isolated from agricultural soils of Kerman Province of Iran and assayed for antagonistic activity against SCLEROTINIA SCLEROTIORUM.10 isolates showed antagonistic effect of In Disc-Agar method, Among selected isolates, STREPTOMYCES isolate 363 UK showed high antagonistic activity. Also, in order to determine genetic diversity of 30 isolates of STREPTOMYCES, the DNA was extracted using CTAB method in laboratory. To do molecular investigation 10 RAPD primers were used for PCR. After doing electrophoresis, 128 sharp bands between 250 and 2800 base pair were recognized. The experiment results were analyzed using NTSYS software and UPGMA method with Dice coefficient. The analyzed cluster found from Dice coefficient divided the 30 STREPTOMYCES isolates in two major groups. In the first group, 10 isolates were antagonistic properties, and in the second group of 20 isolates were no antagonistic effect. Grouping isolates using principal components analysis was performed and two-dimensional and three-dimensional plots respective was drawn. The isolates were divided into eight groups.

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    APA: Copy

    Baniasadi, f., BAGHIZADEH, A., & SHAHIDI BONJAR, GH.H.. (2017). EVALUATION OF ANTAGONISTIC EFFECT AND GENETIC DIVERSITY OF STREPTOMYCES STRAINS ISOLATED FROM SOILS OF THE KERMAN PROVINCE AS BIOLOGICAL CONTROL OF SCLEROTINIA SCLEROTIORUM. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 30(3 ), 357-367. SID. https://sid.ir/paper/248544/en

    Vancouver: Copy

    Baniasadi f., BAGHIZADEH A., SHAHIDI BONJAR GH.H.. EVALUATION OF ANTAGONISTIC EFFECT AND GENETIC DIVERSITY OF STREPTOMYCES STRAINS ISOLATED FROM SOILS OF THE KERMAN PROVINCE AS BIOLOGICAL CONTROL OF SCLEROTINIA SCLEROTIORUM. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2017;30(3 ):357-367. Available from: https://sid.ir/paper/248544/en

    IEEE: Copy

    f. Baniasadi, A. BAGHIZADEH, and GH.H. SHAHIDI BONJAR, “EVALUATION OF ANTAGONISTIC EFFECT AND GENETIC DIVERSITY OF STREPTOMYCES STRAINS ISOLATED FROM SOILS OF THE KERMAN PROVINCE AS BIOLOGICAL CONTROL OF SCLEROTINIA SCLEROTIORUM,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 30, no. 3 , pp. 357–367, 2017, [Online]. Available: https://sid.ir/paper/248544/en

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