THE ROLE OF ASN 28 AND LYS 30 OF AEQUORIN PHOTOPROTEIN IN CALCIUM SENSITIVITY

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ESMAEILI NEZHAD M.; ZEINODDINI M.; MAGHSOUDI N.

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Journal: Journal of Molecular and Cellular Researches Year:2015 | Volume:28 | Issue:4 Page(s): 468-474

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Title

THE ROLE OF ASN 28 AND LYS 30 OF AEQUORIN PHOTOPROTEIN IN CALCIUM SENSITIVITY

Author(s)

ESMAEILI NEZHAD M. | ZEINODDINI M. | MAGHSOUDI N. | Issue Writer Certificate

Keywords

PHOTOPROTEIN AEQUORINQ2

MUTATIONQ1

CALCIUMQ2

SENSITIVITYQ2

Abstract

The photo protein aequorin, isolated from the jellyfishAequoera victoria, is a CALCIUM sensitive bioluminescent protein formed with the apoprotein apoaequorin and the prosthetic group coelenterazine that emits light upon CALCIUM binding. The protein is believed to be a monomer containing four helix-loop-helix motifs (EF hand) of which three domains can bind CALCIUM. On the other hand, excess CALCIUM in brain is associated with many neurodegenerative diseases and conditions including Alzheimer’s, Parkinson’s, and stroke. Aequorin as a natural CALCIUM binding protein taking up excess CALCIUM in brain cells much like a sponge. The aim of this study is production of mutant aequorin for CALCIUM SENSITIVITY increase. The mutated aequorin (N28D, K30T), in EF hand I, were prepared using site directed mutagenesis and subcloned in the pET21 expression vector. According to design of His-taq, purified mutants aequorin prepared using nickel affinity chromatography. Finally CALCIUM SENSITIVITY compared between wild-type and mutant aequorin. CALCIUM SENSITIVITY of mutated aequorin indicated aeq-K30T is more sensitive than wild type and aeq-N28D.

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APA: Copy

ESMAEILI NEZHAD, M., ZEINODDINI, M., & MAGHSOUDI, N.. (2015). THE ROLE OF ASN 28 AND LYS 30 OF AEQUORIN PHOTOPROTEIN IN CALCIUM SENSITIVITY. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(4), 468-474. SID. https://sid.ir/paper/248573/en

Vancouver: Copy

ESMAEILI NEZHAD M., ZEINODDINI M., MAGHSOUDI N.. THE ROLE OF ASN 28 AND LYS 30 OF AEQUORIN PHOTOPROTEIN IN CALCIUM SENSITIVITY. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(4):468-474. Available from: https://sid.ir/paper/248573/en

IEEE: Copy

M. ESMAEILI NEZHAD, M. ZEINODDINI, and N. MAGHSOUDI, “THE ROLE OF ASN 28 AND LYS 30 OF AEQUORIN PHOTOPROTEIN IN CALCIUM SENSITIVITY,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 4, pp. 468–474, 2015, [Online]. Available: https://sid.ir/paper/248573/en

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