OPTIMIZATION OF T DNA TRANSFORMATION AND TRANSGENIC PLANT PRODUCTION OF DATURA METEL L.

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HOMAEI T.; EHSANPOUR A.A.; ASGHARI GH.R.

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Paper Information

Journal: Journal of Molecular and Cellular Researches Year:2015 | Volume:28 | Issue:2 Page(s): 310-317

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Title

OPTIMIZATION OF T DNA TRANSFORMATION AND TRANSGENIC PLANT PRODUCTION OF DATURA METEL L.

Author(s)

HOMAEI T. | EHSANPOUR A.A. | ASGHARI GH.R. | Issue Writer Certificate

Keywords

DATURA METELQ2

ALKALOIDSQ1

TRANSGENICQ2

ATROPINEQ2

AGROBACTERIUMQ2

βGLUCORONIDASEQ1

Abstract

In this study conditions for transformation of DATURA METEL was optimized. T-DNA from pZM1047 containing GUS and NPTII genes was transferred to Datura plant. Bacterial suspension was used for co culture with leaf segments., the they were transferred to MS medium supplemented with 1 mg/L BAP as regenerated medium. After 15-20 days calli with regeneration marks was appeared. The whole regenerated TRANSGENIC plants on kanamycin were checked using PCR. Transformation rate was about 13% which is 2% higher than previous study reported by others.

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APA: Copy

HOMAEI, T., EHSANPOUR, A.A., & ASGHARI, GH.R.. (2015). OPTIMIZATION OF T DNA TRANSFORMATION AND TRANSGENIC PLANT PRODUCTION OF DATURA METEL L.. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(2), 310-317. SID. https://sid.ir/paper/248612/en

Vancouver: Copy

HOMAEI T., EHSANPOUR A.A., ASGHARI GH.R.. OPTIMIZATION OF T DNA TRANSFORMATION AND TRANSGENIC PLANT PRODUCTION OF DATURA METEL L.. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(2):310-317. Available from: https://sid.ir/paper/248612/en

IEEE: Copy

T. HOMAEI, A.A. EHSANPOUR, and GH.R. ASGHARI, “OPTIMIZATION OF T DNA TRANSFORMATION AND TRANSGENIC PLANT PRODUCTION OF DATURA METEL L.,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 2, pp. 310–317, 2015, [Online]. Available: https://sid.ir/paper/248612/en

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