CLONING AND EXPRESSION OF SMTA METALLOTHIONEIN IN ESCHERICHIA COLI FOR HEAVY METAL ABSORPTION

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SAFFAR B.; MOBINI DEHKORDI M.; POULADIAN M.

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Journal: Journal of Molecular and Cellular Researches Year:2015 | Volume:28 | Issue:2 Page(s): 257-265

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Information Journal Paper

Title

CLONING AND EXPRESSION OF SMTA METALLOTHIONEIN IN ESCHERICHIA COLI FOR HEAVY METAL ABSORPTION

Author(s)

SAFFAR B. | MOBINI DEHKORDI M. | POULADIAN M. | Issue Writer Certificate

Keywords

METALLOTHIONEINQ2

SMTAQ2

HEAVY METAL ABSORPTIONQ2

Abstract

One way to absorb heavy metals in addition to chemical methods is the use of biological elements. One of the metal adsorption proteins is METALLOTHIONEIN. Goal: The purpose of this study is gene cloning and expression of Synechococcus PCC7942 METALLOTHIONEIN SMTA and protein production and analysis of metal uptake by the protein. Methods: The gene SMTA was cloned into the T / A cloning vector and then were transferred into the E. coli (DH5a). Colony-PCR evaluates the accuracy of the transformation and sequence detection was carried out. The gene was subcoloned into the expression vector pET15b and transferred into the E. coli (BL21). Gene expression was induced by IPTG and proteins were analyzed by Tris-Trisin SDS-PAGE and Western blotting. Metal uptake of protein was measured by atomic absorption. Results: Sequencing, confirmed the accuracy of the transformation. SDS-PAGE and Western blot analysis confirmed protein expression. Metal absorption showed that one milligram of protein adsorbs 28 nmol of cadmium ion.

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APA: Copy

SAFFAR, B., MOBINI DEHKORDI, M., & POULADIAN, M.. (2015). CLONING AND EXPRESSION OF SMTA METALLOTHIONEIN IN ESCHERICHIA COLI FOR HEAVY METAL ABSORPTION. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(2), 257-265. SID. https://sid.ir/paper/248622/en

Vancouver: Copy

SAFFAR B., MOBINI DEHKORDI M., POULADIAN M.. CLONING AND EXPRESSION OF SMTA METALLOTHIONEIN IN ESCHERICHIA COLI FOR HEAVY METAL ABSORPTION. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(2):257-265. Available from: https://sid.ir/paper/248622/en

IEEE: Copy

B. SAFFAR, M. MOBINI DEHKORDI, and M. POULADIAN, “CLONING AND EXPRESSION OF SMTA METALLOTHIONEIN IN ESCHERICHIA COLI FOR HEAVY METAL ABSORPTION,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 2, pp. 257–265, 2015, [Online]. Available: https://sid.ir/paper/248622/en

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