ROLLING CIRCLE AMPLIFICATION AS A METHOD FOR IDENTIFICATION OF BIRDS (CASE STUDY: ALECTORIS CHUKAR)

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JAVAHERI TEHRANI S.; ALIABADIAN M.; NAJAFZADEH M.J.

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Journal: Journal of Animal Research Year:2017 | Volume:29 | Issue:4 Page(s): 400-410

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Title

ROLLING CIRCLE AMPLIFICATION AS A METHOD FOR IDENTIFICATION OF BIRDS (CASE STUDY: ALECTORIS CHUKAR)

Author(s)

JAVAHERI TEHRANI S. | ALIABADIAN M. | NAJAFZADEH M.J. | Issue Writer Certificate

Keywords

PADLOCK PROBESQ1

ROLLING CIRCLE AMPLIFICATIONQ1

GALLIFORMESQ1

GAME BIRDSQ1

CHUKARQ1

Abstract

The GALLIFORMES are one of the most threatened groups of birds which hunting and other forms of exploitation are applying for different purposes such as: food preparation, entertainment or other commercial purposes. Hunting and over exploitations have been leading that the high percentage of GALLIFORMES species (26.4%) are placed as threatened with extinction in the IUCN Red List. Therefore, in order to take effective conservation actions and preventing of over hunting of GALLIFORMES and also to ensure their survival we introduce the RCA as a certain and quick method to identify the GAME BIRDS by offending hunters. In this method, specific probes are designed for Alectoris chuka r based on differences in nucleotide regions of the cytochrome c oxidase subunit I gene. Specific probes created a closed molecule after hybridization with DNA and were evaluated by attaching to the target and amplification in isothermal condition. The amplification product could be easily visualized on agarose gel electrophoresis or by adding fluorescence staining in UV. In conclusion, regarding the simplicity of RCA reaction and no need to special laboratory instruments, this method provides a strong tool for identification of gamebirds (Chukar and Pheasant) and resolve their management and conservation problems.

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APA: Copy

JAVAHERI TEHRANI, S., ALIABADIAN, M., & NAJAFZADEH, M.J.. (2017). ROLLING CIRCLE AMPLIFICATION AS A METHOD FOR IDENTIFICATION OF BIRDS (CASE STUDY: ALECTORIS CHUKAR). JOURNAL OF ANIMAL RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 29(4), 400-410. SID. https://sid.ir/paper/248765/en

Vancouver: Copy

JAVAHERI TEHRANI S., ALIABADIAN M., NAJAFZADEH M.J.. ROLLING CIRCLE AMPLIFICATION AS A METHOD FOR IDENTIFICATION OF BIRDS (CASE STUDY: ALECTORIS CHUKAR). JOURNAL OF ANIMAL RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2017;29(4):400-410. Available from: https://sid.ir/paper/248765/en

IEEE: Copy

S. JAVAHERI TEHRANI, M. ALIABADIAN, and M.J. NAJAFZADEH, “ROLLING CIRCLE AMPLIFICATION AS A METHOD FOR IDENTIFICATION OF BIRDS (CASE STUDY: ALECTORIS CHUKAR),” JOURNAL OF ANIMAL RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 29, no. 4, pp. 400–410, 2017, [Online]. Available: https://sid.ir/paper/248765/en

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