IN VITRO REGERERATION IN LINUM ALBUM KOTSCHY EX BOISS

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JAVADIAN N.; KARIMZADEH GH.; SHARIFI M.; MOIENI A.

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Journal: Journal of Plant Research Year:2015 | Volume:28 | Issue:4 Page(s): 737-745

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Title

IN VITRO REGERERATION IN LINUM ALBUM KOTSCHY EX BOISS

Author(s)

JAVADIAN N. | KARIMZADEH GH. | SHARIFI M. | MOIENI A. | Issue Writer Certificate

Keywords

LINUM ALBUMQ2

MEDICINAL PLANTQ1

IN VITRO CULTUREQ2

CALLUS INDUCTIONQ2

REGENERATIONQ2

Abstract

LINUM ALBUMof the member Linaceae is a herbaceous Iranian endemic MEDICINAL PLANT, having podophyllotoxin (PTOX) metabolite. This important pharmaceutical compound used as a precursor for the synthesis of important antitumor drugs like Etoposide, Teniposide and Etopophos. Plant tissues culture is an appropriate method of increasing secondary metabolits production. In this research, CALLUS INDUCTION was investigated to REGENERATION underin vitro condition. Calli were initiated from shoot segment of young plant on Murashige and Skoog (MS) culture medium supplemented with (0- 0.4- 0.8- 1-1.5- 2- 2.5 mg l-1) naphthalene acetic acid (NAA) and (0- 0.2- 0.4 mg l-1) kinetin (KIN) in the dark. The heaviest calli were obtained in 2 mg l-1 NAA with 0.4 mg l-1 KIN. Calli were then transferred to MS medium supplemented with 6-benzyladenine (1-2.5 mg l-1 BA) either alone or in combination with 0.2 mg l-1 NAA for shoot REGENERATION. The 2.0 mg l-1 BA was identified more effective on shoot REGENERATION. For induction root from shoots, the optimal result obtained from half-strength MS medium supplemented with 1 mg l-1 NAA.

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APA: Copy

JAVADIAN, N., KARIMZADEH, GH., SHARIFI, M., & MOIENI, A.. (2015). IN VITRO REGERERATION IN LINUM ALBUM KOTSCHY EX BOISS. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(4), 737-745. SID. https://sid.ir/paper/248922/en

Vancouver: Copy

JAVADIAN N., KARIMZADEH GH., SHARIFI M., MOIENI A.. IN VITRO REGERERATION IN LINUM ALBUM KOTSCHY EX BOISS. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(4):737-745. Available from: https://sid.ir/paper/248922/en

IEEE: Copy

N. JAVADIAN, GH. KARIMZADEH, M. SHARIFI, and A. MOIENI, “IN VITRO REGERERATION IN LINUM ALBUM KOTSCHY EX BOISS,” JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 4, pp. 737–745, 2015, [Online]. Available: https://sid.ir/paper/248922/en

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