INVESTIGATION OF EFFECT OF DIFFERENT CONCENTRATIONS OF 2, 4- D AND KINETIN ON CALLUS INDUCTION IN POTATO (SOLANUM TUBEROSUM L.) VARIETY OF AGRIA

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KARAMZAD ZH.; FARSHADFAR M.; ZEBARJADI A.R.; ZOLNORIAN H.

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Journal: Journal of Plant Research Year:2015 | Volume:28 | Issue:2 Page(s): 316-325

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Title

INVESTIGATION OF EFFECT OF DIFFERENT CONCENTRATIONS OF 2, 4- D AND KINETIN ON CALLUS INDUCTION IN POTATO (SOLANUM TUBEROSUM L.) VARIETY OF AGRIA

Author(s)

KARAMZAD ZH. | FARSHADFAR M. | ZEBARJADI A.R. | ZOLNORIAN H. | Issue Writer Certificate

Keywords

POTATOQ2

2Q2

4- DQ2

KINETINQ2

CALLUSQ2

Abstract

Plant tissue culture techniques are a useful tool to increase the genetic diversity, crop production and production of plants virus free. The purpose of this study was reached to a useful method for CALLUS induction. The experiment was set up based on factorial experiment and completely randomized design (CRD) with 4 replications. The effect of different concentrations of 2, 4- D (0, 1, 2, 4 mg/l), Kin (0.25 mg/l) plant growth regulators and two explants (leaf and internode) were evaluated on CALLUS induction. Results of analysis of variance showed that there was significant difference between two types of explants (leaf and internode) for diameter and fresh weight and percentage of CALLUS induction traits, also different concentrations of 2, 4- D and KINETIN were significant for all traits. The interaction of PGR*explant was significant for all traits. The results of means comparison showed that the highest percentage of CALLUS induction (75%) was in leaf explants at a concentration of 2 mg/l (2, 4- D) and for internode explant (87.5%) at 4 mg/l of 2, 4- D. Diameter and fresh weight of CALLUS were maximum in leaf explant (5.60 mm and 0.55 g) and in internode explant (9.13 mm and 0.92 g) at 2 mg/l concentration of 2, 4- D.

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APA: Copy

KARAMZAD, ZH., FARSHADFAR, M., ZEBARJADI, A.R., & ZOLNORIAN, H.. (2015). INVESTIGATION OF EFFECT OF DIFFERENT CONCENTRATIONS OF 2, 4- D AND KINETIN ON CALLUS INDUCTION IN POTATO (SOLANUM TUBEROSUM L.) VARIETY OF AGRIA. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(2), 316-325. SID. https://sid.ir/paper/248955/en

Vancouver: Copy

KARAMZAD ZH., FARSHADFAR M., ZEBARJADI A.R., ZOLNORIAN H.. INVESTIGATION OF EFFECT OF DIFFERENT CONCENTRATIONS OF 2, 4- D AND KINETIN ON CALLUS INDUCTION IN POTATO (SOLANUM TUBEROSUM L.) VARIETY OF AGRIA. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(2):316-325. Available from: https://sid.ir/paper/248955/en

IEEE: Copy

ZH. KARAMZAD, M. FARSHADFAR, A.R. ZEBARJADI, and H. ZOLNORIAN, “INVESTIGATION OF EFFECT OF DIFFERENT CONCENTRATIONS OF 2, 4- D AND KINETIN ON CALLUS INDUCTION IN POTATO (SOLANUM TUBEROSUM L.) VARIETY OF AGRIA,” JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 2, pp. 316–325, 2015, [Online]. Available: https://sid.ir/paper/248955/en

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