EFFECT OF CHITOSAN ON OSTEOGENIC PROPERTIES OF MESENCHYMAL STEM CELL OF EXFOLIATED DECIDUOUS TEETH

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MASOUM TAHEREH; AMIRI IRAJ; RAFATJOU REZVAN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Dental Medicine Year:2013 | Volume:26 | Issue:1 (74) Page(s): 55-63

Download Full-Text

Persian Verion

View:

644

Download:

Cites:

Information Journal Paper

Title

EFFECT OF CHITOSAN ON OSTEOGENIC PROPERTIES OF MESENCHYMAL STEM CELL OF EXFOLIATED DECIDUOUS TEETH

Author(s)

MASOUM TAHEREH | AMIRI IRAJ | RAFATJOU REZVAN | Issue Writer Certificate

Keywords

STEM CELLQ2

DECIDUOUS TOOTHQ2

GLUCOSAMINEQ2

CHITOSANQ2

Abstract

Background and Aims: The exfoliated human DECIDUOUS TOOTH contains multipotent STEM CELLs [STEM CELL from Human Exfoliated DECIDUOUS TOOTH (SHED)] that identified to be a population of highly proliferative and clonogenic. These cells are capable of differentiating into a variety of cell types including osteoblast/osteocyte, adiopcyte, chondrocyte and neural cell. The aim of this study was to evaluate the differentiation of SHED to osteoblast in standard osteogenic medium and comparing the results with medium which supplemented with GLUCOSAMINE in form of CHITOSAN.Materials and Methods: Dental pulp cells were isolated from freshly extracted primary teeth, digested with 4 mg/ml collogenase/dispase, and grown in Dulbecco's modified Eagle's medium with 10 percent fetal bovine serum. The clonogenic potential of cells was performed after 3 weeks of culture. Flowcytometric analysis, performed at day 21 of culture to identify surface markers of mesenchymal STEM CELLs. The cells from 3rd passage used for osteogenic differentiation in routine osteoinductive medium. CHITOSAN (10 mg/ml) was added to the culture medium of case group. Alizarin Red Staining and Alkaline Phosphatase (ALP) activity were done to evaluate osteogenic differentiation in the developing adherent layer on the third passage. The results were analyzed using T-test. For the analysis of normal distribution of data, non-parametric Kolmogrov-Smirnov test was used.Results: The colonogenic efficiency was more than 80%. Flowcytometric analysis showed that expression of mesenchymal STEM CELL marker CD90, CD105 and CD146 were positive in SHED, while hematopoietic cell marker CD34, CD45 and endothelial cell marker CD31 were negative. Quantitative analysis of Alizarin Red Staining demonstrated that: mineralized nodule formation was higher in the group supplemented with GLUCOSAMINE (chitosan). Results from Alkaline Phosphatase activity test, on day 21, demonstrated a significantly higher ALP activity in the group supplemented CHITOSAN (P<0.001).Conclusion: STEM CELLs isolated and cultured from exfoliated deciduous teeth pulp, can be differentiated to osteoblast. Addition of CHITOSAN can be beneficial to promote osteogenic differentiation of these cells.

Cites

No record.

References

No record.

Cite

APA: Copy

MASOUM, TAHEREH, AMIRI, IRAJ, & RAFATJOU, REZVAN. (2013). EFFECT OF CHITOSAN ON OSTEOGENIC PROPERTIES OF MESENCHYMAL STEM CELL OF EXFOLIATED DECIDUOUS TEETH. JOURNAL OF DENTAL MEDICINE, 26(1 (74)), 55-63. SID. https://sid.ir/paper/25451/en

Vancouver: Copy

MASOUM TAHEREH, AMIRI IRAJ, RAFATJOU REZVAN. EFFECT OF CHITOSAN ON OSTEOGENIC PROPERTIES OF MESENCHYMAL STEM CELL OF EXFOLIATED DECIDUOUS TEETH. JOURNAL OF DENTAL MEDICINE[Internet]. 2013;26(1 (74)):55-63. Available from: https://sid.ir/paper/25451/en

IEEE: Copy

TAHEREH MASOUM, IRAJ AMIRI, and REZVAN RAFATJOU, “EFFECT OF CHITOSAN ON OSTEOGENIC PROPERTIES OF MESENCHYMAL STEM CELL OF EXFOLIATED DECIDUOUS TEETH,” JOURNAL OF DENTAL MEDICINE, vol. 26, no. 1 (74), pp. 55–63, 2013, [Online]. Available: https://sid.ir/paper/25451/en

Related Journal Papers