EXPRESSION OF LEAF PROTEINS IN TWO CULTIVARS OF BREAD WHEAT UNDER CADMIUM AND MERCURY STRESS USING TWO-DIMENSIONAL GEL ELECTROPHORESIS

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RAEESI SADATI S.Y.; JAHANBAKHSH GODEKAHRIZ S.; EBADI A.

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Journal: JOURNAL OF CROP PRODUCTION AND PROCESSING Year:2016 | Volume:5 | Issue:18 Page(s): 233-244

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Title

EXPRESSION OF LEAF PROTEINS IN TWO CULTIVARS OF BREAD WHEAT UNDER CADMIUM AND MERCURY STRESS USING TWO-DIMENSIONAL GEL ELECTROPHORESIS

Author(s)

RAEESI SADATI S.Y. | JAHANBAKHSH GODEKAHRIZ S. | EBADI A. | Issue Writer Certificate

Keywords

ELECTROPHORESQ3

PROTEINQ2

HEAVY METALSQ2

WHEATQ3

Abstract

Wheat is an important source of human food. Cadmium and mercury bind to sulfhydryl groups of structural PROTEINs and enzymes and cause inhibition in activity and decrease in PROTEIN production or interfere with the regulation of the enzymes. To study the effect of PROTEIN expression under different levels of cadmium and mercury, the experiment was conducted in a completely randomized design with three replications in Mohaghegh Ardabili University, Ardabil, Iran. Experimental factors consisted of two Gonbad and Tajan bread what cultivars, HEAVY METALS in seven levels (four concentrations of mercuric chloride in 5, 10, 15 and 20 µM and cadmium chloride at two concentrations of 0.25 and 0.5 mM) and sampling time after 8 and 16 hours of treatment. The Bradford method was used for quantitative analysis of PROTEINs and 12% SDS-PAGE and two dimensional ELECTROPHORESe techniques were hired for analysis of their expression. The results showed that under cadmium and mercury stresses, the total PROTEIN content increased compared to the control. Two-dimensional ELECTROPHORESis of PROTEINs under cadmium stress showed differential expression of the PROTEIN spots on the plant leaves, than the control. In general, changes in the expression of PROTEINs under the effect of cadmium stress were divided into two main categories: Spots 9, 10, 13, 14 and 16 belonged to PROTEINs with reduced expression and the spots 1, 2, 8, 19 and 20 belonged to PROTEINs with increased expression, in comparison to non-stressed control. These spots of up regulated PROTEINs were directly related to the defense system against the heavy metal stress.

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APA: Copy

RAEESI SADATI, S.Y., JAHANBAKHSH GODEKAHRIZ, S., & EBADI, A.. (2016). EXPRESSION OF LEAF PROTEINS IN TWO CULTIVARS OF BREAD WHEAT UNDER CADMIUM AND MERCURY STRESS USING TWO-DIMENSIONAL GEL ELECTROPHORESIS. JOURNAL OF CROP PRODUCTION AND PROCESSING, 5(18), 233-244. SID. https://sid.ir/paper/255852/en

Vancouver: Copy

RAEESI SADATI S.Y., JAHANBAKHSH GODEKAHRIZ S., EBADI A.. EXPRESSION OF LEAF PROTEINS IN TWO CULTIVARS OF BREAD WHEAT UNDER CADMIUM AND MERCURY STRESS USING TWO-DIMENSIONAL GEL ELECTROPHORESIS. JOURNAL OF CROP PRODUCTION AND PROCESSING[Internet]. 2016;5(18):233-244. Available from: https://sid.ir/paper/255852/en

IEEE: Copy

S.Y. RAEESI SADATI, S. JAHANBAKHSH GODEKAHRIZ, and A. EBADI, “EXPRESSION OF LEAF PROTEINS IN TWO CULTIVARS OF BREAD WHEAT UNDER CADMIUM AND MERCURY STRESS USING TWO-DIMENSIONAL GEL ELECTROPHORESIS,” JOURNAL OF CROP PRODUCTION AND PROCESSING, vol. 5, no. 18, pp. 233–244, 2016, [Online]. Available: https://sid.ir/paper/255852/en

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