PREVALENCE OF β-LACTAMASES GENES OF IMP, SHV AND PER IN PSEUDOMONAS AERUGINOSA ISOLATED FROM HOSPITALS IN KERMANSHAH

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SALIMI AFSANEH; AKYA ALISHA; HAIDARI ERFAN

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Journal: Journal of Clinical Research in Paramedical Sciences Year:2015 | Volume:4 | Issue:2 Page(s): 152-159

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Title

PREVALENCE OF β-LACTAMASES GENES OF IMP, SHV AND PER IN PSEUDOMONAS AERUGINOSA ISOLATED FROM HOSPITALS IN KERMANSHAH

Author(s)

SALIMI AFSANEH | AKYA ALISHA | HAIDARI ERFAN | Issue Writer Certificate

Keywords

PSEUDOMONAS AERUGINOSAQ2

β-LACTAMASESQ2

PERQ2

SHVQ2

IMPQ2

Abstract

Background: PSEUDOMONAS AERUGINOSA is an opportunistic pathogen associated with nosocomial infections. The emergence and dissemination of metallo-b-lactamases (MBLs) and extended-spectrum- b -lactamases (ESBLs) have contributed to the high rate of resistance among the p. aeruginosa isolates. This study investigated the prevalence of IMP, SHV and PER genes in p. aeruginosa isolates.Methods: Antibiotic susceptibility testing was PERformed using the minimal inhibitory concentrations (MIC). Then the polymerase chain reaction (PCR) was used for the detection of 3 genes encoding IMP, SHV and PER.Results: Of the 60 isolates tested, 38 (63.3%) were resistant to piperacilin, 42 (70%) to ceftazidime, 44 (73.3%) to imipenem and 45 (75%) to cefepime. Five (8.3%) and 8 (13.3%) of the isolates had PER and SHV genes by PCR results. In this study IMP gene was not found.Conclusion: According to this study, P. aeruginosa isolates were highly resistant to antibiotics. Therefore the susceptibility of isolates should be tested before treatment. Among the three b -lactamases genes studied the highest prevalence was related to SHV gene. Given the clinical significance of MBLs and ESBLs producing isolates, identification of these organisms is essential in the hospitals in order to get a better therapeutic response and control of bacterial dissemination.

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APA: Copy

SALIMI, AFSANEH, AKYA, ALISHA, & HAIDARI, ERFAN. (2015). PREVALENCE OF β-LACTAMASES GENES OF IMP, SHV AND PER IN PSEUDOMONAS AERUGINOSA ISOLATED FROM HOSPITALS IN KERMANSHAH. JOURNAL OF CLINICAL RESEARCH IN PARAMEDICAL SCIENCES, 4(2), 152-159. SID. https://sid.ir/paper/256102/en

Vancouver: Copy

SALIMI AFSANEH, AKYA ALISHA, HAIDARI ERFAN. PREVALENCE OF β-LACTAMASES GENES OF IMP, SHV AND PER IN PSEUDOMONAS AERUGINOSA ISOLATED FROM HOSPITALS IN KERMANSHAH. JOURNAL OF CLINICAL RESEARCH IN PARAMEDICAL SCIENCES[Internet]. 2015;4(2):152-159. Available from: https://sid.ir/paper/256102/en

IEEE: Copy

AFSANEH SALIMI, ALISHA AKYA, and ERFAN HAIDARI, “PREVALENCE OF β-LACTAMASES GENES OF IMP, SHV AND PER IN PSEUDOMONAS AERUGINOSA ISOLATED FROM HOSPITALS IN KERMANSHAH,” JOURNAL OF CLINICAL RESEARCH IN PARAMEDICAL SCIENCES, vol. 4, no. 2, pp. 152–159, 2015, [Online]. Available: https://sid.ir/paper/256102/en

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