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Information Journal Paper

Title

CRYOPRESERVATION OF SMIRNOVIA IRANICA (SABETI) SEEDS AND EVALUATION OF CRYOPRESERVED SEEDS UNDER LABORATORY, GREENHOUSE AND NATURAL HABITAT CONDITIONS

Pages

  122-137

Abstract

SMIRNOVIA IRANICA(Sabeti) synonym: SMIRNOVIA TURKESTANA (Bunge) is a deciduous perennial bushy species of the Fabaceae (Papilionaceae) family. The species grow on sand dunes of desert areas, having a deep vertical root and very long horizontal or lateral roots. They have an important role in natural vegetation of desert and sand dune stabilization. Limited growing areas, grazing due to good palatability, and foliage quality of the plant have put this species under threat. Seed preservation under cryogenic conditions at -196ºC is an important approach to conserve seeds for a long period. In this study, seeds of theS. iranica were collected from natural habitats of the plants and three pre-cryopreservation treatments including PVS2, DESICCATION, and 30%Glycerol were applied before transferring the seeds into liquid nitrogen (LN) at -196ºC for 1 week, 1 month, and 1 year (in 2015). Subsequently, the seeds were removed from the liquid nitrogen, imposed to heat shock and evaluated under laboratory, greenhouse and natural habitat conditions. The cryopreserved seeds of various cryopreservation periods germinated normally under laboratory and greenhouse conditions. In laboratory conditions, there were no significant differences between periods of seed storage in cryogenic conditions for SEED GERMINATION trait. The highest SEED GERMINATION percent (84%) was observed in the DESICCATION pre-cryopreservation treatment. In pre-cryopreservation treatments as well as cryogenic storage periods under greenhouse conditions, SEED GERMINATION and seedling establishment were significantly different. In natural habitat, the cryopreserved seeds germinate and grow to normal seedlings and plants. The results showed thatS. iranica seeds can be successfully stored in cryogenic conditions for a long period.

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    APA: Copy

    NADERI SHAHAB, MOHABAT ALI, JEBELLI, MARYAM, & ASHRAF JAFARI, ALI. (2017). CRYOPRESERVATION OF SMIRNOVIA IRANICA (SABETI) SEEDS AND EVALUATION OF CRYOPRESERVED SEEDS UNDER LABORATORY, GREENHOUSE AND NATURAL HABITAT CONDITIONS. JOURNAL OF RANGELAND SCIENCE, 7(2), 122-137. SID. https://sid.ir/paper/257567/en

    Vancouver: Copy

    NADERI SHAHAB MOHABAT ALI, JEBELLI MARYAM, ASHRAF JAFARI ALI. CRYOPRESERVATION OF SMIRNOVIA IRANICA (SABETI) SEEDS AND EVALUATION OF CRYOPRESERVED SEEDS UNDER LABORATORY, GREENHOUSE AND NATURAL HABITAT CONDITIONS. JOURNAL OF RANGELAND SCIENCE[Internet]. 2017;7(2):122-137. Available from: https://sid.ir/paper/257567/en

    IEEE: Copy

    MOHABAT ALI NADERI SHAHAB, MARYAM JEBELLI, and ALI ASHRAF JAFARI, “CRYOPRESERVATION OF SMIRNOVIA IRANICA (SABETI) SEEDS AND EVALUATION OF CRYOPRESERVED SEEDS UNDER LABORATORY, GREENHOUSE AND NATURAL HABITAT CONDITIONS,” JOURNAL OF RANGELAND SCIENCE, vol. 7, no. 2, pp. 122–137, 2017, [Online]. Available: https://sid.ir/paper/257567/en

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