CLONING AND SEQUENCING OF XYLANASE OFANOXYBACILLUS FLAVITHERMUSFROM QINARJEH, MESHKIN-SHAHR, IRAN

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MOKHTARPOUR NAEIMEH; AGHAMAALI MAHMOUD REZA; ASGHARI SEYED MOHSEN

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Journal: AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY Year:2015 | Volume:3 | Issue:2 Page(s): 15-30

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Title

CLONING AND SEQUENCING OF XYLANASE OFANOXYBACILLUS FLAVITHERMUSFROM QINARJEH, MESHKIN-SHAHR, IRAN

Author(s)

MOKHTARPOUR NAEIMEH | AGHAMAALI MAHMOUD REZA | ASGHARI SEYED MOHSEN | Issue Writer Certificate

Keywords

ANOXYBACILLUS FLAVITHERMUSQ2

CLONINGQ2

PCRQ2

XYLANASEQ2

Abstract

The thermostable and alkaliphile XYLANASEs are beneficial industrial enzymes in food industry (bakery and alcohol production or fermentation industries) pulp-kraft, and pharmaceutic industry. XYLANASEs hydrolyse xylan (the hemicellulose of plant cell wall) and produced by different kinds of microorganisms like bacteria, fungi and some algae. In this study, XYLANASE gene from thermoalkaline ANOXYBACILLUS FLAVITHERMUS was isolated from hot spring of Meshkinshahr (Iran) and was cloned in E. coli (BL21). At first, total DNA was extracted from bacteria. PCR was then accomplished and XYLANASE gene was isolated and amplified. Amplification of xylenase coding gene was confirmed by electrophoresis. CLONING was performed by digestion of XYLANASE gene and pET21a+ by restriction enzymes (NheI and XhoI), following by ligation and transformation to E. coli (BL21). Finally, the results were confirmed by colony-PCR and sequencing of selected colonies. PCR and sequencing results, demonstrated a high similarity between XYLANASEs of Anoxybacillus species with XYLANASE of this study.

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APA: Copy

MOKHTARPOUR, NAEIMEH, AGHAMAALI, MAHMOUD REZA, & ASGHARI, SEYED MOHSEN. (2015). CLONING AND SEQUENCING OF XYLANASE OFANOXYBACILLUS FLAVITHERMUSFROM QINARJEH, MESHKIN-SHAHR, IRAN. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, 3(2), 15-30. SID. https://sid.ir/paper/261287/en

Vancouver: Copy

MOKHTARPOUR NAEIMEH, AGHAMAALI MAHMOUD REZA, ASGHARI SEYED MOHSEN. CLONING AND SEQUENCING OF XYLANASE OFANOXYBACILLUS FLAVITHERMUSFROM QINARJEH, MESHKIN-SHAHR, IRAN. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY[Internet]. 2015;3(2):15-30. Available from: https://sid.ir/paper/261287/en

IEEE: Copy

NAEIMEH MOKHTARPOUR, MAHMOUD REZA AGHAMAALI, and SEYED MOHSEN ASGHARI, “CLONING AND SEQUENCING OF XYLANASE OFANOXYBACILLUS FLAVITHERMUSFROM QINARJEH, MESHKIN-SHAHR, IRAN,” AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, vol. 3, no. 2, pp. 15–30, 2015, [Online]. Available: https://sid.ir/paper/261287/en

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