CYP1A GENE SEQUENCING AND STUDY OF GENE EXPRESSIONPATTERN IN DIFFERENT TISSUES OF MUDSKIPPER (PERIOPHTHALMUSWALTONI) CAPTURED FROM NATURAL HABITAT

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GHASEMI SEYED AHMAD; MOVAHEDINIA ABDOLALI; SALAMAT NEGIN; KAZEMI BAHRAM

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Journal: AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY Year:2017 | Volume:5 | Issue:1 Page(s): 69-86

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Title

CYP1A GENE SEQUENCING AND STUDY OF GENE EXPRESSIONPATTERN IN DIFFERENT TISSUES OF MUDSKIPPER (PERIOPHTHALMUSWALTONI) CAPTURED FROM NATURAL HABITAT

Author(s)

GHASEMI SEYED AHMAD | MOVAHEDINIA ABDOLALI | SALAMAT NEGIN | KAZEMI BAHRAM | Issue Writer Certificate

Keywords

CYTOCHROME P450Q1

ECOPHYSIOLOGYQ2

BIOMARKERQ1

PERIOPHTHALMUS WALTONIQ1

Abstract

Cytochrome CYP1A is one of the main involved enzymes in the metabolism ofthe xenobiotic. Identifying the CYTOCHROME P450 (CYP1A), responsible for themajor metabolic pathways, can lead to understanding the metabolism pathwayof organic materials and PAHs adaptation of sea fish. Results from cloning andidentifying CYP1A gene in PERIOPHTHALMUS WALTONI showed CYP1A contained1035 nucleotide from open reading frame sequenced. CYP1A basal geneexpression was varied from 23, 22, 19, 17, 11, 11% for gill, kidney, heart, intestine, brain respectively. According to the results, liver organ showed the highest response of CYP1A gene expression like other fish species. But, the basic gene expression of CYP1A in other organs in comparison with liver organ was higher. Although basal expression pattern of genes depending on the species, high basal expression of gene CYP1A due to P. waltoni was exposure to PAH in natural conditions. Different CYP1A gene expression pattern indifferent organs showed eco physiological condition of mudskipper.So, P. waltoni has the potential to be used as an exact BIOMARKER for evaluation of organic pollutant of ecosystems especially PAHs.

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APA: Copy

GHASEMI, SEYED AHMAD, MOVAHEDINIA, ABDOLALI, SALAMAT, NEGIN, & KAZEMI, BAHRAM. (2017). CYP1A GENE SEQUENCING AND STUDY OF GENE EXPRESSIONPATTERN IN DIFFERENT TISSUES OF MUDSKIPPER (PERIOPHTHALMUSWALTONI) CAPTURED FROM NATURAL HABITAT. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, 5(1), 69-86. SID. https://sid.ir/paper/261310/en

Vancouver: Copy

GHASEMI SEYED AHMAD, MOVAHEDINIA ABDOLALI, SALAMAT NEGIN, KAZEMI BAHRAM. CYP1A GENE SEQUENCING AND STUDY OF GENE EXPRESSIONPATTERN IN DIFFERENT TISSUES OF MUDSKIPPER (PERIOPHTHALMUSWALTONI) CAPTURED FROM NATURAL HABITAT. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY[Internet]. 2017;5(1):69-86. Available from: https://sid.ir/paper/261310/en

IEEE: Copy

SEYED AHMAD GHASEMI, ABDOLALI MOVAHEDINIA, NEGIN SALAMAT, and BAHRAM KAZEMI, “CYP1A GENE SEQUENCING AND STUDY OF GENE EXPRESSIONPATTERN IN DIFFERENT TISSUES OF MUDSKIPPER (PERIOPHTHALMUSWALTONI) CAPTURED FROM NATURAL HABITAT,” AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, vol. 5, no. 1, pp. 69–86, 2017, [Online]. Available: https://sid.ir/paper/261310/en

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