DEVELOPING A NEW METHOD FOR ISOLATION OF TOTAL PETROLEUM HYDROCARBONS (TPH) FROM PLANTS WITH COLUMN CHROMATOGRAPHY AND DETERMINATION WITH SPECTROFLUOROPHOTOMETRY

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RAVANBAKHSH SH.; FARAHANI A.

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Journal: Nashrieh Shimi va Mohandesi Shimi Iran Year:2007 | Volume:26 | Issue:2 Page(s): 119-123

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Title

DEVELOPING A NEW METHOD FOR ISOLATION OF TOTAL PETROLEUM HYDROCARBONS (TPH) FROM PLANTS WITH COLUMN CHROMATOGRAPHY AND DETERMINATION WITH SPECTROFLUOROPHOTOMETRY

Author(s)

RAVANBAKHSH SH. | FARAHANI A. | Issue Writer Certificate

Keywords

PLANTQ3

TOTAL PETROLEUM HYDROCARBONS (TPH)Q3

COLUMN CHROMATOGRAPHYQ3

MICROWAVE EXTRACTION SPECTROFLUOROPHOTOMETRYQ3

Abstract

The efficiencies of total petroleum hydrocarbons (TPH) transfer into various solvents from PLANTs by microwave enhanced process were extracted. Microwave extraction - realized at 120°C for 30 min with Hexane-Acetone (3:2 V/V) as the extraction solvent was identified as the most effective extraction procedure for isolation of TPH from biotic matrices. The aim of this research is to develop a silica gel and alumina fractionation procedure for PLANT sample extraction. COLUMN CHROMATOGRAPHY with two solvents (chloroform and hexane: dichloromethane) as a mobile phase were used for clean-up of extract. In this research the efficiency of recovery received from chloroform as a mobile phase. With this mobile phase color compounds and carotenoids separated with 15 ml of chloroform (first fraction) and the second fraction with 20 ml chloroform contained total petroleum hydrocarbons. At the end spectrofluorophotometry was employed for quantification of analytes.

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APA: Copy

RAVANBAKHSH, SH., & FARAHANI, A.. (2007). DEVELOPING A NEW METHOD FOR ISOLATION OF TOTAL PETROLEUM HYDROCARBONS (TPH) FROM PLANTS WITH COLUMN CHROMATOGRAPHY AND DETERMINATION WITH SPECTROFLUOROPHOTOMETRY. NASHRIEH SHIMI VA MOHANDESI SHIMI IRAN (PERSIAN), 26(2), 119-123. SID. https://sid.ir/paper/26329/en

Vancouver: Copy

RAVANBAKHSH SH., FARAHANI A.. DEVELOPING A NEW METHOD FOR ISOLATION OF TOTAL PETROLEUM HYDROCARBONS (TPH) FROM PLANTS WITH COLUMN CHROMATOGRAPHY AND DETERMINATION WITH SPECTROFLUOROPHOTOMETRY. NASHRIEH SHIMI VA MOHANDESI SHIMI IRAN (PERSIAN)[Internet]. 2007;26(2):119-123. Available from: https://sid.ir/paper/26329/en

IEEE: Copy

SH. RAVANBAKHSH, and A. FARAHANI, “DEVELOPING A NEW METHOD FOR ISOLATION OF TOTAL PETROLEUM HYDROCARBONS (TPH) FROM PLANTS WITH COLUMN CHROMATOGRAPHY AND DETERMINATION WITH SPECTROFLUOROPHOTOMETRY,” NASHRIEH SHIMI VA MOHANDESI SHIMI IRAN (PERSIAN), vol. 26, no. 2, pp. 119–123, 2007, [Online]. Available: https://sid.ir/paper/26329/en

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