Evaluation type of explants and season of sampling under different disinfection treatments for the tissu culture of Buxus hyrcana Pojark

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Mohamadzade A.; PAYAM NOOR V.; KAVOSI M.R.

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Journal: FOREST RESEARCH AND DEVELOPMENT Year:2020 | Volume:5 | Issue:4 Page(s): 527-540

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Title

Evaluation type of explants and season of sampling under different disinfection treatments for the tissu culture of Buxus hyrcana Pojark

Author(s)

Mohamadzade A. | PAYAM NOOR V. | KAVOSI M.R. | Issue Writer Certificate

Keywords

BoxblightQ1

Treatments of sterilizationQ1

Buxus hyrcana PojarkQ1

Tissu cultureQ2

Abstract

The aim of this study was to find the best explants and sampling period under different sterilization treatments in order to achieve a suitable and optimal protocol for tissue culture studies in Buxus sp. Explants were prepared from different parts of the plant, including leaves and stems. At first, detergent disinfection treatments and initial washing with ordinary water containing a few drops of liquid dish for four to five minutes were performed to remove surface contamination. The Explants were flushed with distilled water five times. Then, under laminar Floe sterilization, use 13 different treatments and disinfect five times each treatment with distilled autoclaved leachate and at the end of the appropriate samples (leaf samples of 0. 5 to 1 cm Squares and stem samples of 5 to 10 mm) were isolated from plant material and placed on a PDA medium inside autoclaved petri dishes alongside a flame. In order to measure the contamination, cultures in a growth room with a temperature of 26 ± 1 ° C with a light intensity of 1000 to 1500 lux and dark (incubator) were investigated. The results showed that samples of spring season have less microbial contamination than those in the autumn season. On the other hand, leaf samples showed less contamination percentage compared to stem explants.

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APA: Copy

Mohamadzade, A., PAYAM NOOR, V., & KAVOSI, M.R.. (2020). Evaluation type of explants and season of sampling under different disinfection treatments for the tissu culture of Buxus hyrcana Pojark. FOREST RESEARCH AND DEVELOPMENT, 5(4 ), 527-540. SID. https://sid.ir/paper/263748/en

Vancouver: Copy

Mohamadzade A., PAYAM NOOR V., KAVOSI M.R.. Evaluation type of explants and season of sampling under different disinfection treatments for the tissu culture of Buxus hyrcana Pojark. FOREST RESEARCH AND DEVELOPMENT[Internet]. 2020;5(4 ):527-540. Available from: https://sid.ir/paper/263748/en

IEEE: Copy

A. Mohamadzade, V. PAYAM NOOR, and M.R. KAVOSI, “Evaluation type of explants and season of sampling under different disinfection treatments for the tissu culture of Buxus hyrcana Pojark,” FOREST RESEARCH AND DEVELOPMENT, vol. 5, no. 4 , pp. 527–540, 2020, [Online]. Available: https://sid.ir/paper/263748/en

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