DETECTION AND ISOLATION OF CD59 FROM HUMAN SEMINAL PLASMA

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

REZAEI A.; NASRESFAHANI MOHAMMAD HOSSEIN; HASHEMI TABAR MAHMOUD; BAHRAMIAN H.; ORAIZI F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS) Year:2002 | Volume:7 | Issue:2 Page(s): 165-169

Download Full-Text

Persian Verion

View:

1,022

Download:

Cites:

Information Journal Paper

Title

DETECTION AND ISOLATION OF CD59 FROM HUMAN SEMINAL PLASMA

Author(s)

Keywords

C059Q4

COMPLEMENT REGULATORY PROTEINQ4

SEMINAL PLASMAQ4

Abstract

Introduction. CD59 is one of the COMPLEMENT REGULATORY PROTEINs (CRPs) which exert inhibitory function by blocking the formation of C5b-9 complex or Membrane Attacks complex (MAC). Regarding the therapeutic role of CD59 in treatment of pathological effects in uncontrolled activation of complements system and its efficiency to overcome the hyper-acute rejection, CD59 was suggested for maintenance of transplanted organ. In this study We determined and isolated CD59 from SEMINAL PLASMA.Methods. Six normospermic sample according to WHO standards were chosen. Plasma of samples was separated and to remove the postasomes, the seminal plama was ultra centrifuged. CD59 was detected by Dot-Blot using CD59 mAb (MEM43). The molecular weight and purity of protein was detected by SOS-PAGE method follwed by Westerm Blot.Results. Protein was present in the 6.5 ml and 15ml of gel fitration fractions. Molecular weight based on marker size in these two fractions was 65 and 21KD respectively. Discussion. CD59 had previously beem purified by lysis of erythrocyte cell membrane. Because of use of detergent and preservative agents, this method decreased physiologic effects of the protein. In this study the isolation was performed from prostasome granules" without using of any detergent and preservative agents.

Cites

No record.

References

No record.

Cite

APA: Copy

REZAEI, A., NASRESFAHANI, MOHAMMAD HOSSEIN, HASHEMI TABAR, MAHMOUD, BAHRAMIAN, H., & ORAIZI, F.. (2002). DETECTION AND ISOLATION OF CD59 FROM HUMAN SEMINAL PLASMA. JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS), 7(2), 165-169. SID. https://sid.ir/paper/26775/en

Vancouver: Copy

REZAEI A., NASRESFAHANI MOHAMMAD HOSSEIN, HASHEMI TABAR MAHMOUD, BAHRAMIAN H., ORAIZI F.. DETECTION AND ISOLATION OF CD59 FROM HUMAN SEMINAL PLASMA. JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS)[Internet]. 2002;7(2):165-169. Available from: https://sid.ir/paper/26775/en

IEEE: Copy

A. REZAEI, MOHAMMAD HOSSEIN NASRESFAHANI, MAHMOUD HASHEMI TABAR, H. BAHRAMIAN, and F. ORAIZI, “DETECTION AND ISOLATION OF CD59 FROM HUMAN SEMINAL PLASMA,” JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS), vol. 7, no. 2, pp. 165–169, 2002, [Online]. Available: https://sid.ir/paper/26775/en

Related Journal Papers