Fast protein liquid chromatography

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Rahimifard Mahshid; YOUSEFI MARYAM; NADERI MAHMOUD

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF LABORATORY KNOWLEDGE Year:2018 | Volume:6 | Issue:3 (23) Page(s): 15-21

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Information Journal Paper

Title

Fast protein liquid chromatography

Author(s)

Rahimifard Mahshid | YOUSEFI MARYAM | NADERI MAHMOUD | Issue Writer Certificate

Keywords

fast protein liquid chromatography

ion exchange

gel filtration

affinity

hydrophobic interaction

Abstract

fast protein liquid chromatography (FPLC) is a form of medium pressure chromatography originally developed for purifying proteins with high resolution and reproducibility. Its distinguishing feature is that the stationary phase is composed of small-diameter beads (generally cross-linked agarose) that are packed in glass or plastic columns and have high loading capacity. The FPLC system allows the use of a wide range of aqueous buffers (the mobile phase) and different stationary phases to perform the main chromatography modes (ion exchange, gel filtration, affinity and hydrophobic interaction, reverse phase). However, anion exchange and gel filtration chromatography are the modes most commonly used.

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APA: Copy

Rahimifard, Mahshid, YOUSEFI, MARYAM, & NADERI, MAHMOUD. (2018). Fast protein liquid chromatography. IRANIAN JOURNAL OF LABORATORY KNOWLEDGE, 6(3 (23) ), 15-21. SID. https://sid.ir/paper/269061/en

Vancouver: Copy

Rahimifard Mahshid, YOUSEFI MARYAM, NADERI MAHMOUD. Fast protein liquid chromatography. IRANIAN JOURNAL OF LABORATORY KNOWLEDGE[Internet]. 2018;6(3 (23) ):15-21. Available from: https://sid.ir/paper/269061/en

IEEE: Copy

Mahshid Rahimifard, MARYAM YOUSEFI, and MAHMOUD NADERI, “Fast protein liquid chromatography,” IRANIAN JOURNAL OF LABORATORY KNOWLEDGE, vol. 6, no. 3 (23) , pp. 15–21, 2018, [Online]. Available: https://sid.ir/paper/269061/en

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