Preparing of biological samples for observing with Scanning Electron Microscope (SEM)

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KHaninour Maryam; Rasouli Masoud; Hashemian Nafiseh

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Paper Information

Journal: IRANIAN JOURNAL OF LABORATORY KNOWLEDGE Year:2019 | Volume:7 | Issue:1 (25) Page(s): 13-26

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Title

Preparing of biological samples for observing with Scanning Electron Microscope (SEM)

Author(s)

KHaninour Maryam | Rasouli Masoud | Hashemian Nafiseh | Issue Writer Certificate

Keywords

Scanning Electron Microscope (SEM)

biological samples

Live Tissues

sample preparation

Abstract

electron microscope is one of the best methods of analysis that is widely used in various fields. The microscope provides the possibility of examining the surface and microstructure in micron and nano dimensions. Today, one of the most specialized areas of interest is biological and living tissue. The observation of these samples and the investigation of their microstructures by scanning electron microscope require special preparation. Due to the high sensitivity of the samples, if any of the steps in the preparation are not carefully performed, it will cause shrinkage, collapse, or damage to the sample structure and will affect the quality of the final images, so it is very important that the conditions and procedures for tissue preparation carefully done with the standard method. There are many different techniques for preparing and analyzing various types of tissue that vary according to the type of tissue and structure. This paper study the general methods for the preparation of biological samples and living tissues including: a) various fixations (chemical fixtaion and physical fixation), (b) dehydration with different percentages of alcohol, and (c) different drying methods In air, critical point dryers, freeze dryers and chemical dryers), in order to view the biological structures in the closest to their living structure in a high resolution SEM is discussed.

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APA: Copy

KHaninour, Maryam, Rasouli, Masoud, & Hashemian, Nafiseh. (2019). Preparing of biological samples for observing with Scanning Electron Microscope (SEM). IRANIAN JOURNAL OF LABORATORY KNOWLEDGE, 7(1 (25) ), 13-26. SID. https://sid.ir/paper/269065/en

Vancouver: Copy

KHaninour Maryam, Rasouli Masoud, Hashemian Nafiseh. Preparing of biological samples for observing with Scanning Electron Microscope (SEM). IRANIAN JOURNAL OF LABORATORY KNOWLEDGE[Internet]. 2019;7(1 (25) ):13-26. Available from: https://sid.ir/paper/269065/en

IEEE: Copy

Maryam KHaninour, Masoud Rasouli, and Nafiseh Hashemian, “Preparing of biological samples for observing with Scanning Electron Microscope (SEM),” IRANIAN JOURNAL OF LABORATORY KNOWLEDGE, vol. 7, no. 1 (25) , pp. 13–26, 2019, [Online]. Available: https://sid.ir/paper/269065/en

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