Isolation and investigation of eugenol O-methyl transferase (EOMTs) gene promoter from Ocimum basilicum L.

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Khakdan Fatemeh; RANJBAR MOJTABA; CHAPARZADEH NADER; Javanmard Athar Sadat; ALIZADEH HOUSHANG

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Journal: GENETIC ENGINEERING AND BIOSAFETY JOURNAL Year:2019 | Volume:7 | Issue:2 Page(s): 189-202

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Title

Isolation and investigation of eugenol O-methyl transferase (EOMTs) gene promoter from Ocimum basilicum L.

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Keywords

EOMTs promoter

cis-acting elements

drought stress

Ocimum basilicum L

Abstract

Ocimum basilicum L. (2n=48) is a medicinal plant that belongs to Lamiaceae family. Eugenol is one of the most important components of basil essential oil which is utilized as an anti-bacterial and anti-fungal material in various industries. Phenylpropanoids such as methyl eugenol possess anti-oxidant properties against free radicals and act as protective compounds in plant responses to the biotic and abiotic stresses. Methyl eugenol O-methyl transferase (EOMTs), as a key enzyme of phenylpropanoids biosynthesis pathway, converts eugenol into methyl eugenol by methylation. In this study, we isolated EOMTs gene promoter from the basil plant and cloned in pTG19-T vector for sequencing. Then, this sequence was characterized and analyzed by bioinformatics softwares. We aimed to investigate the possible gene expression regulation of EOMTs gene under different environmental conditions and recognition different regulatory motifs of its promoter. Analysis of EOMTs gene promoter (1209 bp) indicated that this sequence included some important regulatory elements which could respond to high temperature and drought stress such as MYB, MYC, HSE, and WRKY. Transient expression of the GUS gene under ObEOMTs promoter control in Nicotiana tabacum leaves showed that this promoter including core elements was able to direct the basal expression of this gene. It is expected that this promoter will be capable of changing the expression levels of EOMTs gene in reaction to different environmental stresses because there are stress-responsive regulatory sites in ObEOMTs gene promoter.

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APA: Copy

Khakdan, Fatemeh, RANJBAR, MOJTABA, CHAPARZADEH, NADER, Javanmard, Athar Sadat, & ALIZADEH, HOUSHANG. (2019). Isolation and investigation of eugenol O-methyl transferase (EOMTs) gene promoter from Ocimum basilicum L.. GENETIC ENGINEERING AND BIOSAFETY JOURNAL, 7(2 ), 189-202. SID. https://sid.ir/paper/270504/en

Vancouver: Copy

Khakdan Fatemeh, RANJBAR MOJTABA, CHAPARZADEH NADER, Javanmard Athar Sadat, ALIZADEH HOUSHANG. Isolation and investigation of eugenol O-methyl transferase (EOMTs) gene promoter from Ocimum basilicum L.. GENETIC ENGINEERING AND BIOSAFETY JOURNAL[Internet]. 2019;7(2 ):189-202. Available from: https://sid.ir/paper/270504/en

IEEE: Copy

Fatemeh Khakdan, MOJTABA RANJBAR, NADER CHAPARZADEH, Athar Sadat Javanmard, and HOUSHANG ALIZADEH, “Isolation and investigation of eugenol O-methyl transferase (EOMTs) gene promoter from Ocimum basilicum L.,” GENETIC ENGINEERING AND BIOSAFETY JOURNAL, vol. 7, no. 2 , pp. 189–202, 2019, [Online]. Available: https://sid.ir/paper/270504/en

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