Phylogenetic analysis and genetic variation of Tomato yellow leaf curl virus based on the V1 gene in Iraq

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Al Waeli Mahnad; DIZADJI AKBAR; Mossahebi Gholam Hossein; Ahangaran Akbar

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Journal: GENETIC ENGINEERING AND BIOSAFETY JOURNAL Year:2018 | Volume:7 | Issue:1 Page(s): 1-11

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Title

Phylogenetic analysis and genetic variation of Tomato yellow leaf curl virus based on the V1 gene in Iraq

Author(s)

Al Waeli Mahnad | DIZADJI AKBAR | Mossahebi Gholam Hossein | Ahangaran Akbar | Issue Writer Certificate

Keywords

Begomovirus

Genetic diversity

Iraq

Tomato

Abstract

Tomato yellow leaf curl virus (TYLCV) is a supreme pathogen in tropical and subtropical areas. During 2014-2015, a total of 393 Tomato leaf tissue samples showing Tomato yellow leaf curl disease (TYLCD) symptoms were collected from six different provinces of Iraq. In double antibody sandwich enzymelinked imunoserbent assay (DAS-ELSA) 55 out of 393 samples (14%) reacted positively with TYLCVspecific antibodies. Following total DNA extraction, the presence of TYLCV was verified in 21 out of 55 ELISA positive samples by PCR and coat protein gene (V1) of 16 TYLCV isolates was amplified using specific primers and cloned. Nucleotide sequences of Iraqi TYLCV isolates of V1 gene showed the highest nucleotide sequences identity of 94. 1-99. 6% in CP gene with Kuwait (KJ830841) and Iraq (JQ354991) isolates. In phylogenetic analysis based on V1 nucleotide sequences, Iraqi isolates were separated into two main groups and four subgroups, without correlation with geographical origin. Genetic diversity parameters based on V1 nucleotide sequences indicated a high genetic variability in Iraqi populations of TYLCV. The proportion of non-synonymous to synonymous nucleotide diversity was <1. 0, indicating that this gene is under negative selection. A low gene flow was observed between southern and centric Iraq subpopulations, demonstrating genetic differentiation among Iraqi subpopulations. This is the first study dealing with the distribution and genetic variation of TYLCV in Iraq. Fulllength viral genome sequencing of TYLCV isolates of Iraq is necessary to differentiate virus strain(s) in Iraq.

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APA: Copy

Al Waeli, Mahnad, DIZADJI, AKBAR, Mossahebi, Gholam Hossein, & Ahangaran, Akbar. (2018). Phylogenetic analysis and genetic variation of Tomato yellow leaf curl virus based on the V1 gene in Iraq. GENETIC ENGINEERING AND BIOSAFETY JOURNAL, 7(1 ), 1-11. SID. https://sid.ir/paper/270546/en

Vancouver: Copy

Al Waeli Mahnad, DIZADJI AKBAR, Mossahebi Gholam Hossein, Ahangaran Akbar. Phylogenetic analysis and genetic variation of Tomato yellow leaf curl virus based on the V1 gene in Iraq. GENETIC ENGINEERING AND BIOSAFETY JOURNAL[Internet]. 2018;7(1 ):1-11. Available from: https://sid.ir/paper/270546/en

IEEE: Copy

Mahnad Al Waeli, AKBAR DIZADJI, Gholam Hossein Mossahebi, and Akbar Ahangaran, “Phylogenetic analysis and genetic variation of Tomato yellow leaf curl virus based on the V1 gene in Iraq,” GENETIC ENGINEERING AND BIOSAFETY JOURNAL, vol. 7, no. 1 , pp. 1–11, 2018, [Online]. Available: https://sid.ir/paper/270546/en

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