ISOLATION OF BRUCELLA ABORTUS USING PCR-RFLP ANALYSIS

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SALEHI MANSOUR; SALEHI R.; PISHVA E.; RAHMANI M.

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Paper Information

Journal: Iranian Journal of Public Health Year:2006 | Volume:35 | Issue:4 Page(s): 22-27

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Information Journal Paper

Title

ISOLATION OF BRUCELLA ABORTUS USING PCR-RFLP ANALYSIS

Author(s)

SALEHI MANSOUR | SALEHI R. | PISHVA E. | RAHMANI M. | Issue Writer Certificate

Keywords

BRUCELLA ABORTUS

PCR-RFLP

OMP2A

IRAN

Abstract

Brucella transmission and epidemiology depend on infecting species and biovar. Therefore, exact identification of the Brucella is important to design correct control and treatment strategies. In this study, we examined presence of otherBrucellae in Isfahan. One hundred twenty Brucella isolates were collected and genomic DNA was extracted from them. OMP2A fragment of all isolates were amplified using a pair of specific primers and the PCR products were lectrophoresed and stained with EtBr. These PCR products were then restricted using PstI restriction endonuclease. The PCR products of all isolates had the same size of 1100bp. The banding pattern of PCR-RFLP for all of the isolates were similar to banding pattern of the Brucella melitensis biotype 1 except for 5 samples that demonstrated banding pattern similar to B. abortus. Based on our results, it is clear that biotype 1 of the B. melitensis is not the only Brucella present in Isfahan and now B. abortus is also present in our area. These results are very important in planning for the control of the disease as well epidemiology and even treatment of the patients.

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APA: Copy

SALEHI, MANSOUR, SALEHI, R., PISHVA, E., & RAHMANI, M.. (2006). ISOLATION OF BRUCELLA ABORTUS USING PCR-RFLP ANALYSIS. IRANIAN JOURNAL OF PUBLIC HEALTH, 35(4), 22-27. SID. https://sid.ir/paper/272358/en

Vancouver: Copy

SALEHI MANSOUR, SALEHI R., PISHVA E., RAHMANI M.. ISOLATION OF BRUCELLA ABORTUS USING PCR-RFLP ANALYSIS. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2006;35(4):22-27. Available from: https://sid.ir/paper/272358/en

IEEE: Copy

MANSOUR SALEHI, R. SALEHI, E. PISHVA, and M. RAHMANI, “ISOLATION OF BRUCELLA ABORTUS USING PCR-RFLP ANALYSIS,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 35, no. 4, pp. 22–27, 2006, [Online]. Available: https://sid.ir/paper/272358/en

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