EFFECTS OF ESTROGEN AND PROGESTERONE ON ENDOMETRIAL GLYCOCALYX EXPRESSION IN SUPEROVULATED MICE IN THE IMPLANTATION WINDOW TIME

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NIKNAFS B.; AFSHAR FATEMEH

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Journal: IRANIAN JOURNAL OF ENDOCRINOLOGY AND METABOLISM (IJEM) Year:2009 | Volume:10 | Issue:6 (SN 42) Page(s): 639-646

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Title

EFFECTS OF ESTROGEN AND PROGESTERONE ON ENDOMETRIAL GLYCOCALYX EXPRESSION IN SUPEROVULATED MICE IN THE IMPLANTATION WINDOW TIME

Author(s)

NIKNAFS B. | AFSHAR FATEMEH | Issue Writer Certificate

Keywords

IMPLANTATIONQ3

OVARIAN HYPERSTIMULATIONQ3

GLYCOCALYXQ2

ENDOMETRIUMQ3

Abstract

Introduction: It has been reported that apical GLYCOCALYX expression of uterine affects embryo IMPLANTATION. The aim of this study was to determine endometrial GLYCOCALYX and endometrial thickness after estrogen and progesterone injection in hyperstimulated mice at luteal phase.Materials & Methods: Adult male and female mice were used for induction of pesudopregnancy. The mice were divided into two groups; experimental and control groups. Female mice in the experimental group were superovulated and were then mated with vasectomised mice to induce psudopregnancy; Experimental group based on hormone injection was subdivided into five groups; 1) Estrogen, 2) Progesterone, 3) Estrogen + Progesterone, 4) Antiprogesterone + Estrogen, 5) Sham. Pesudopregnancy in the control group was achieved without any hyperstimulation. The uterines of all groups were collected after 4.5 days of pregnancy and prepared for the assessment of endometrial thickness and endometrial GLYCOCALYX expression. Results: The results showed that estrogen and progesterone injection increased the intensity of PAS reaction, whereas progesterone decreased this. Also our results showed that the endometrial thickness in the sham group was the highest and in the progesterone group was the lowest. Conclusion: Our results showed that addition of estrogen to progesterone, compared to P supplementation alone, provided appropriate endometrial conditions to embryo IMPLANTATION. Hence combination of estrogen and progesterone injection as luteal support hormones can be used in IVF protocols.

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APA: Copy

NIKNAFS, B., & AFSHAR, FATEMEH. (2009). EFFECTS OF ESTROGEN AND PROGESTERONE ON ENDOMETRIAL GLYCOCALYX EXPRESSION IN SUPEROVULATED MICE IN THE IMPLANTATION WINDOW TIME. IRANIAN JOURNAL OF ENDOCRINOLOGY AND METABOLISM (IJEM), 10(6 (SN 42)), 639-646. SID. https://sid.ir/paper/27238/en

Vancouver: Copy

NIKNAFS B., AFSHAR FATEMEH. EFFECTS OF ESTROGEN AND PROGESTERONE ON ENDOMETRIAL GLYCOCALYX EXPRESSION IN SUPEROVULATED MICE IN THE IMPLANTATION WINDOW TIME. IRANIAN JOURNAL OF ENDOCRINOLOGY AND METABOLISM (IJEM)[Internet]. 2009;10(6 (SN 42)):639-646. Available from: https://sid.ir/paper/27238/en

IEEE: Copy

B. NIKNAFS, and FATEMEH AFSHAR, “EFFECTS OF ESTROGEN AND PROGESTERONE ON ENDOMETRIAL GLYCOCALYX EXPRESSION IN SUPEROVULATED MICE IN THE IMPLANTATION WINDOW TIME,” IRANIAN JOURNAL OF ENDOCRINOLOGY AND METABOLISM (IJEM), vol. 10, no. 6 (SN 42), pp. 639–646, 2009, [Online]. Available: https://sid.ir/paper/27238/en

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