A NEW PRIMER PAIR IN ITS1 REGION FOR MOLECULAR STUDIES ON ECHINOCOCCUS GRANULOSUS

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RAHIMI H.R.; KIA E.B.; MIRHENDI S.H.; TALEBI A.; FASIHI HARANDI M.; JALALIZAND N.; ROKNI M.B.

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Journal Paper

Paper Information

Journal: Iranian Journal of Public Health Year:2007 | Volume:36 | Issue:1 Page(s): 45-49

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Title

A NEW PRIMER PAIR IN ITS1 REGION FOR MOLECULAR STUDIES ON ECHINOCOCCUS GRANULOSUS

Author(s)

Keywords

ECHINOCOCCUS GRANULOSUS

PCR

PRIMER DESIGN

DNA

ITS1

DNASIS

Abstract

Background: Echinocuccus granulosus, the causative agent of cystic echinococcosis has long been recognized as having a high degree of genetic divergence. The strains characterization seems to be essential for the establishment of a preventiveand control strategy in every endemic area. Using DNA based methods for strain /genotype characterizations of E. granulosus have some difficulties, especially access to an efficient and pure concentration of DNA and proper primers. Methods: Using grinder method, a pure and high concentration DNA was extracted from 10 human hydatid cysts collected from Isfahan (central Iran) hospitals, and processed for PCR reaction. Results: Using DNASIS, the primers were designed in internal transcribed spacer 1 (ITS1) region, following analysis of 30 E. granulosus nucleotide sequences, extracted from gene bank.Conclusion: This new and specific E. granulosus primer which amplified DNA thoroughly can be applied for molecular studies on echinococcosis.

Cites

COMPARISON OF FIVE SIMPLE METHODS FOR DNA EXTRACTION FROM ECHINOCOCCUS GRANULOSUS PROTOSCOLECES FOR PCR AMPLIFICATION OF RIBOSOMAL DNA

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APA: Copy

RAHIMI, H.R., KIA, E.B., MIRHENDI, S.H., TALEBI, A., FASIHI HARANDI, M., JALALIZAND, N., & ROKNI, M.B.. (2007). A NEW PRIMER PAIR IN ITS1 REGION FOR MOLECULAR STUDIES ON ECHINOCOCCUS GRANULOSUS. IRANIAN JOURNAL OF PUBLIC HEALTH, 36(1), 45-49. SID. https://sid.ir/paper/272405/en

Vancouver: Copy

RAHIMI H.R., KIA E.B., MIRHENDI S.H., TALEBI A., FASIHI HARANDI M., JALALIZAND N., ROKNI M.B.. A NEW PRIMER PAIR IN ITS1 REGION FOR MOLECULAR STUDIES ON ECHINOCOCCUS GRANULOSUS. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2007;36(1):45-49. Available from: https://sid.ir/paper/272405/en

IEEE: Copy

H.R. RAHIMI, E.B. KIA, S.H. MIRHENDI, A. TALEBI, M. FASIHI HARANDI, N. JALALIZAND, and M.B. ROKNI, “A NEW PRIMER PAIR IN ITS1 REGION FOR MOLECULAR STUDIES ON ECHINOCOCCUS GRANULOSUS,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 36, no. 1, pp. 45–49, 2007, [Online]. Available: https://sid.ir/paper/272405/en

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