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Information Journal Paper

Title

Molecular Phylogenetic Variability of Fasciola gigantica in Iran

Pages

  740-747

Abstract

 Background: Fascioliasis is one of important zoonotic disease caused by Fasciola gigantica and F. hepatica. The final hosts of this parasite are ruminants and humans. Iran is one of the endemic areas in the world, about six million people at risk of infection. The aim of this study was to identify and determine the Genetic diversity of Fasciola species in cattle after distinguish of their species. Methods: One hundred and seventeen liver specimens collected from naturally infected cattle in 5 geograph-ical regions in 2014-2017. Flukes stained with Hematoxylin-Carmine dye to examine for the existence of sperm within seminal vesicles. DNA was extracted from each individual, and ITS1, ND1and CO1 genes were ampli-fied using specific primers. For discrimination of Fasciola species, ITS1 PCR-RFLP was used based on diges-tion pattern of RsaI enzyme. Genetic analyses and diversity and neutrality indices estimated by Dnasp5 based on NDI. Results: Six nonspermic and 111 spermic flukes were diagnosed. All of nonspermic specimens were F. gigantica and collected from South East, South West and North West of Iran. Genetic haplotype diversity has been observed in F. gigantica based on ND1. Fst value analysis showed that minimum and maximum genetic difference between Iranian F. gigantica with Bangladesh (Fst = 0. 01414) and Egypt (Fst = 0. 36653) respectively. Conclusion: It is the first report of existing of nonspermic Fasciola. High haplotype and nucleotide diversity could be due to ecological factors in life cycle, animal migration and coexisting of the final host of this para-site. Haplotype and nucleotide diversity of spermic F. gigantica in Iran and other countries in the world led to creating a variety of haplogroups.

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    APA: Copy

    RAEGHI, SABER, ROUHANI, SOHEILA, FASIHI HARANDI, MAJID, SPOTIN, ADEL, & GHODSIAN, sahar. (2019). Molecular Phylogenetic Variability of Fasciola gigantica in Iran. IRANIAN JOURNAL OF PUBLIC HEALTH, 48(4), 740-747. SID. https://sid.ir/paper/275129/en

    Vancouver: Copy

    RAEGHI SABER, ROUHANI SOHEILA, FASIHI HARANDI MAJID, SPOTIN ADEL, GHODSIAN sahar. Molecular Phylogenetic Variability of Fasciola gigantica in Iran. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2019;48(4):740-747. Available from: https://sid.ir/paper/275129/en

    IEEE: Copy

    SABER RAEGHI, SOHEILA ROUHANI, MAJID FASIHI HARANDI, ADEL SPOTIN, and sahar GHODSIAN, “Molecular Phylogenetic Variability of Fasciola gigantica in Iran,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 48, no. 4, pp. 740–747, 2019, [Online]. Available: https://sid.ir/paper/275129/en

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