AFFINITY-BASED TARGET DECONVOLUTION OF SAFRANAL

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HOSSEINZADEH HOSSEIN; MEHRI SOGHRA; ABOLHASSANI MOHAMMAD MAHDI; RAMEZANI MOHAMMAD; SAHEBKAR AMIRHOSSEIN; ABNOUS KHALIL

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Journal Paper

Paper Information

Journal: DARU Journal of Pharmaceutical Sciences Year:2013 | Volume:21 | Issue:3 Page(s): 0-0

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Title

AFFINITY-BASED TARGET DECONVOLUTION OF SAFRANAL

Author(s)

Keywords

SAFRANAL

CROCUS SATIVUS

SAFFRON

TARGET DECONVOLUTION

AFFINITY CHROMATOGRAPHY

PROTEOMICS

Abstract

Background and the purpose of the study: Affinity-based TARGET DECONVOLUTION is an emerging method for the identification of interactions between drugs/drug candidates and cellular proteins, and helps to predict potential activities and side effects of a given compound. In the present study, we hypothesized that a part of SAFRANAL pharmacological effects, one of the major constituent of CROCUS SATIVUS L., relies on its physical interaction with target proteins.Methods: AFFINITY CHROMATOGRAPHY solid support was prepared by covalent attachment of SAFRANAL to agarose beads.After passing tissue lysate through the column, SAFRANAL-bound proteins were isolated and separated on SDS-PAGE or two-dimensional gel electrophoresis. Proteins were identified using MALDI-TOF/TOF mass spectrometry and Mascot software.Results and major conclusion: Data showed that SAFRANAL physically binds to beta actin, cytochrome b-c1 complex sub-unit 1, trifunctional enzyme sub-unit beta and ATP synthase sub-unit alpha and beta. These interactions may explain part of SAFRANAL’s pharmacological effects. However, phenotypic and/or biological relevance of these interactions remains to be elucidated by future pharmacological studies.

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APA: Copy

HOSSEINZADEH, HOSSEIN, MEHRI, SOGHRA, ABOLHASSANI, MOHAMMAD MAHDI, RAMEZANI, MOHAMMAD, SAHEBKAR, AMIRHOSSEIN, & ABNOUS, KHALIL. (2013). AFFINITY-BASED TARGET DECONVOLUTION OF SAFRANAL. DARU JOURNAL OF PHARMACEUTICAL SCIENCE, 21(3), 0-0. SID. https://sid.ir/paper/275675/en

Vancouver: Copy

HOSSEINZADEH HOSSEIN, MEHRI SOGHRA, ABOLHASSANI MOHAMMAD MAHDI, RAMEZANI MOHAMMAD, SAHEBKAR AMIRHOSSEIN, ABNOUS KHALIL. AFFINITY-BASED TARGET DECONVOLUTION OF SAFRANAL. DARU JOURNAL OF PHARMACEUTICAL SCIENCE[Internet]. 2013;21(3):0-0. Available from: https://sid.ir/paper/275675/en

IEEE: Copy

HOSSEIN HOSSEINZADEH, SOGHRA MEHRI, MOHAMMAD MAHDI ABOLHASSANI, MOHAMMAD RAMEZANI, AMIRHOSSEIN SAHEBKAR, and KHALIL ABNOUS, “AFFINITY-BASED TARGET DECONVOLUTION OF SAFRANAL,” DARU JOURNAL OF PHARMACEUTICAL SCIENCE, vol. 21, no. 3, pp. 0–0, 2013, [Online]. Available: https://sid.ir/paper/275675/en

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