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Information Journal Paper

Title

OPTIMIZATION OF THE STATIC HUMAN OSTEOBLAST/OSTEOCLAST CO-CULTURE SYSTEM

Pages

  208-213

Abstract

 Osteoblasts (OBs) and OSTEOCLASTS (OCs) are 2 major groups of bone cells. Their cell-to-cell interactions are important to ensure the continuity of the bone-remodeling process. Therefore, the present study was carried out to optimize an OB/OC co-culture system utilizing the human OB cell line hFOB 1.19 and OCs extracted from peripheral blood mononuclear cells (PBMNCs).It was a 2-step procedure, involving the optimization of the OB culture and the co-culture of the OBs with PBMNCs at an optimum ratio. Firstly, pre-OBs were cultured to 90% confluency and the time required for differentiation was determined. OB differentiation was determined using the van Gieson staining to detect the presence of collagen and Alizarin Red for calcium.Secondly, OBs and OCs were co-cultured at the ratios of 1 OC: 1 OB, 1 OC: 4 OBs, 2 OCs: 1 OB, and 1 OC: 2 OBs. Tartrateresistant acid phosphatase (TRAP) staining was used to detect the differentiation of the OCs. The results showed that collagen was present on day 1, whereas calcium was detected as early as day 3. Based on the result of TRAP staining, 1 OC: 2 OBs was taken as the most appropriate ratio. No macrophage colonystimulating factor and receptor activator of the nuclear factor-κB ligand were added because they were provided by the OBs. In conclusion, these optimization processes are vital as they ensure the exact time point and ratio of the OB/OC co-culture in order to produce a reliable and reproducible co-culture system.

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    APA: Copy

    JOLLY, JAMES JAM, CHIN, KOK YONG, NUR FARHANA, MOHD FOZI, ALIAS, EKRAM, CHUA, KIEN HUI, WAN HASAN, WAN NURAINI, & IMA NIRWANA, SOELAIMAN. (2018). OPTIMIZATION OF THE STATIC HUMAN OSTEOBLAST/OSTEOCLAST CO-CULTURE SYSTEM. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), 43(2), 208-213. SID. https://sid.ir/paper/276575/en

    Vancouver: Copy

    JOLLY JAMES JAM, CHIN KOK YONG, NUR FARHANA MOHD FOZI, ALIAS EKRAM, CHUA KIEN HUI, WAN HASAN WAN NURAINI, IMA NIRWANA SOELAIMAN. OPTIMIZATION OF THE STATIC HUMAN OSTEOBLAST/OSTEOCLAST CO-CULTURE SYSTEM. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS)[Internet]. 2018;43(2):208-213. Available from: https://sid.ir/paper/276575/en

    IEEE: Copy

    JAMES JAM JOLLY, KOK YONG CHIN, MOHD FOZI NUR FARHANA, EKRAM ALIAS, KIEN HUI CHUA, WAN NURAINI WAN HASAN, and SOELAIMAN IMA NIRWANA, “OPTIMIZATION OF THE STATIC HUMAN OSTEOBLAST/OSTEOCLAST CO-CULTURE SYSTEM,” IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), vol. 43, no. 2, pp. 208–213, 2018, [Online]. Available: https://sid.ir/paper/276575/en

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